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Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking Pubmed



Larijani B1 ; Aghayan H2 ; Goodarzi P3 ; Mohamadijahani F4 ; Norouzijavidan A5 ; Dehpour AR6 ; Fallahzadeh K2 ; Sayahpour FA7 ; Bidaki K4 ; Arjmand B8
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Source: Acta Medica Iranica Published:2015


Abstract

In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials. © 2015 Tehran University of Medical Sciences. All rights reserved.
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