Differential Expression of Micrornas in Renal Transplant Patients With Acute T-Cell Mediated Rejection Publisher Pubmed



Soltaninejad E^{1, 2} ; Nicknam MH^{2, 3} ; Nafar M⁴ ; Ahmadpoor P⁴ ; Pourrezagholi F⁴ ; Sharbafi MH² ; Hosseinzadeh M² ; Foroughi F³ ; Yekaninejad MS⁵ ; Bahrami T³ ; Sharifpaghaleh E² ; Amirzargar A^{2, 3} Show Affiliations
Source: Transplant Immunology Published:2015

Abstract

Background: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA). Methods: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA. Results: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients. Conclusion: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA. © 2015 Elsevier B.V.