Enhancement of Cisplatin-Induced Apoptosis by Saffron in Human Lung Cancer Cells Publisher Pubmed



Mokhtari Z¹ ; Seyedhashemi E² ; Eftekhari M³ ; Ghasemi S⁴ ; Sabouri A⁵ ; Abbaszadehgoudarzi K⁶ ; Abuali M⁷ ; Azimi H⁸ ; Kesharwani P^{9, 10} ; Pourghadamyari H^{11, 12, 13} ; Sahebkar A^{14, 15, 16} Show Affiliations
Source: Journal of Trace Elements in Medicine and Biology Published:2023

Abstract

Background: Cisplatin is a prevalent chemotherapeutic agent, and it has been used extensively to treat lung cancer. However, its clinical efficacy is hampered by its safety profile and dose-limiting toxicity. Saffron is a natural product that has shown significant anticancer effects. The combination treatment of saffron with chemotherapeutic agents has been considered a new strategy. Methods: Herein, saffron extract as a natural anticancer substance was combined with cisplatin to assess their combined efficacy against tumor development in vitro. In A549 and QU-DB cell lines, the combined effect of the saffron extract with cisplatin led to a significant reduction in cell viability as compared to cisplatin alone. Results: After 48 h incubation a considerable reduction in ROS levels in the QU-DB cell line upon treatment with cisplatin in the presence of saffron extract in comparison with cells treated with cisplatin alone. Furthermore, apoptosis increased significantly when in cells treated with cisplatin in combination with saffron extract compared to cisplatin alone. Conclusion: Our data establish that the combination of saffron extract as a natural anticancer substance with cisplatin leads to improved cell toxicity of cisplatin as an anticancer agent. Therefore, the saffron extract could be potentially used as an additive to enable a reduction in cisplatin dosages and its side effects. © 2023 Elsevier GmbH