Cytoprotective Effects of Olesoxime on Isolated Human Pancreatic Islets in Order to Attenuate Apoptotic Pathway Publisher Pubmed



Kaviani M¹ ; Keshtkar S^{1, 2} ; Azarpira N¹ ; Aghdaei MH¹ ; Geramizadeh B¹ ; Karimi MH¹ ; Shamsaeefar A³ ; Motazedian N¹ ; Nikeghbalian S³ ; Alabdullah IH⁴ ; Ghahremani MH^{2, 5} Show Affiliations
Source: Biomedicine and Pharmacotherapy Published:2019

Abstract

Background and purpose: Islet transplantation is considered as a promising approach in the treatment of diabetes type 1. In this regard, optimal culture of the pancreatic islets is promising in the success of transplantation. In the present study, the effect of olesoxime, as an antiapoptotic substance, was evaluated on human islet culture. Experimental approach: The pancreatic islets were isolated by mechanical and enzymatic techniques. After overnight recovery, the islets were treated by different concentrations of olesoxime for 24 and 72 h. Then, they were examined in terms of viability, apoptosis, genes and proteins expression including BAX, BCL2, active caspase-3, and insulin. Moreover, the islets function was evaluated through the glucose-induced insulin and C-peptide secretion assay. Key results: Our findings showed that the islets increased in apoptosis and the decreased in viability after 72 h; also, insulin and C-peptide secretion reduced. However, in the presence of olesoxime, BAX/BCL2 ratio and the activation of caspase-3 were decreased. Therefore, olesoxime could improve the viability of the islets with the decrease of apoptosis. Conclusion: The application of olesoxime can reduce the stressful condition for the islets in vitro and subsequently improve their viability and functionality. © 2019 The Authors