Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold

Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold Publisher Pubmed

Mansour RN¹ ; Barati G² ; Soleimani M³ ; Ghoraeian P⁴ ; Nouri Aleagha M⁴ ; Kehtari M⁵ ; Mahboudi H⁶ ; Hosseini F⁷ ; Hassannia H⁸ ; Abazari MF⁴ ; Enderami SE¹

Show Affiliations

1. Stem Cell Technology Research Center, Tehran, Iran
2. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
5. School of Biology, College of Sciences, University of Tehran, Tehran, Iran
6. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
8. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018

Abstract

Transplantation of islet is a promising method in treatment of patients with type 1 diabetes mellitus (T1DM), however, is limited by islet shortage. The aim of this study was to prepare a polyethersulfone (PES) nanofibrous scaffolds to evaluate the pancreatic differentiation of human induced pluripotent stem cells (hiPSCs). The differentiation process in tissue culture dishes and PES scaffolds was evaluated at mRNA and protein level by RT-qPCR and immunofluorescence assay, respectively. The functionality of differentiated cells was determined by insulin and C-peptide release in response to glucose challenges. The results of this study showed that cells cultured on PES nanofibrous scaffolds exhibit more pancreatic β-cell characteristics as they express more pancreatic tissue-specific genes and proteins. Furthermore, the immunoassay showed that differentiated cells in both culture plates and PES scaffolds groups are functional and secrete C-peptide and insulin in response to glucose challenges. Altogether, the results of this study demonstrated that PES nanofibrous scaffold could provide the microenvironment that promotes the differentiation of induced pluripotent stem cells (iPSCs) into insulin producing cells. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

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Style	Citing Format
MLA	Mansour RN, et al.. "Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold." Artificial Cells# Nanomedicine and Biotechnology, vol. 46, no. sup1, 2018, pp. 733-739.
APA	Mansour RN, Barati G, Soleimani M, Ghoraeian P, Nouri Aleagha M, Kehtari M, Mahboudi H, Hosseini F, Hassannia H, Abazari MF, Enderami SE (2018). Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold. Artificial Cells# Nanomedicine and Biotechnology, 46(sup1), 733-739.
Chicago	Mansour RN, Barati G, Soleimani M, Ghoraeian P, Nouri Aleagha M, Kehtari M, Mahboudi H, et al.. "Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold." Artificial Cells# Nanomedicine and Biotechnology 46, no. sup1 (2018): 733-739.
Harvard	Mansour RN et al. (2018) 'Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold', Artificial Cells# Nanomedicine and Biotechnology, 46(sup1), pp. 733-739.
Vancouver	Mansour RN, Barati G, Soleimani M, Ghoraeian P, Nouri Aleagha M, Kehtari M, et al.. Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold. Artificial Cells# Nanomedicine and Biotechnology. 2018;46(sup1):733-739.
BibTex	@article{ author = {Mansour RN and Barati G and Soleimani M and Ghoraeian P and Nouri Aleagha M and Kehtari M and Mahboudi H and Hosseini F and Hassannia H and Abazari MF and Enderami SE}, title = {Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {46}, number = {sup1}, pages = {733-739}, year = {2018} }
RIS	TY - JOUR AU - Mansour RN AU - Barati G AU - Soleimani M AU - Ghoraeian P AU - Nouri Aleagha M AU - Kehtari M AU - Mahboudi H AU - Hosseini F AU - Hassannia H AU - Abazari MF AU - Enderami SE TI - Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold JO - Artificial Cells# Nanomedicine and Biotechnology VL - 46 IS - sup1 SP - 733 EP - 739 PY - 2018 ER -

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