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Inherent Anti-Hiv Activity of Biocompatible Anionic Citrate-Peg-Citrate Dendrimer Publisher Pubmed



Kandi MR1 ; Mohammadnejad J1 ; Shafiee Ardestani M2 ; Zabihollahi R3 ; Soleymani S3 ; Aghasadeghi MR3 ; Baesi K3
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran
  2. 2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Hepatitis and AIDS Department, Pasteur Institute of Iran, P. O. Box 14115-331, Tehran, Iran

Source: Molecular Biology Reports Published:2019


Abstract

The development of new combinations to empower better protection against HIV infection is particularly important. Anionic polymers can block HIV infection. In the current study, first generation (G1) and second generation (G2) novel water-soluble anionic citrate-PEG-citrate dendrimers were synthesized and characterized with Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), and dynamic light scattering (DLS) methods. After the biocompatibility of the G2 dendrimer was determined, its antiviral activity was evaluated. This function may contribute to the peripheral groups of this dendrimer (carboxylate group). In order to measure the inhibitory effect of G2 on HIV infection, both pre-treatment (treated with G2 dendrimer before HIV infection) and co-treatment (simultaneously treated with G2 dendrimer and HIV infection) were used in vitro. The results showed the good synthesis of the G2 dendrimer, and the dendrimer showed antiviral properties (ICC50:0.4 mM) and low toxicity (CC50:0.6 mM) at high concentrations. A strong inhibitory effect was found when the co-treatment approach was used. This study achieved promising results which encourage the use of G2 dendrimers as anti-HIV agents. © 2018, Springer Nature B.V.