A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps

A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps Publisher

Khashayar P^{1, 2, 3} ; Amoabediny G^{4, 5} ; Larijani B⁶ ; Hosseini M¹ ; Verplancke R² ; De Keersmaecker M⁷ ; Adriaens A⁷ ; Goemaere S⁸ ; Fiers T⁸ ; Vanfleteren J²

Show Affiliations

1. Nanobiotechnology Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2. Center for Microsystems Technology, IMEC and Ghent University, Zwijnaarde, Ghent, Belgium
3. Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, Tehran, Iran
5. Nanobiotechnology Department, Research Center for New Technology in Life Sciences Engineering, University of Tehran, Tehran, Iran
6. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute (EMRI), Shariati Hospital, Tehran University of Medical Sciences, Kargar St, Tehran, Iran
7. Department of Analytical Chemistry, Ghent University, Krijgslaan 281 (S12), Ghent, Belgium
8. Unit for Osteoporosis and Metabolic Bone Diseases, Ghent University Hospital, Ghent, Belgium

Source: 3 Biotech Published:2017

Abstract

This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation) and can thus be used for point of care screening. Due to the excellent electrical properties of the as-prepared immunosensor, CTX levels were successfully measured from 1 to 1000 pg/mL. This is while the normal reference of the marker is 50–450 pg/mL, suggesting that the sensor can acceptably detect CTX. The results also showed a good correlation with ECLIA in measuring serum levels of CTX. © 2017, Springer-Verlag GmbH Germany.

2. A Multiplexed Microfluidic Platform for Bone Marker Measurement: A Proof-Of-Concept, Micromachines (2017)

3. Fabrication and Verification of Conjugated Aunp-Antibody Nanoprobe for Sensitivity Improvement in Electrochemical Biosensors, Scientific Reports (2017)

4. Protocol for Preliminary, Multicenteric Validation of Pocosteo Device: A Point of Care Tool for Proteomic and Genomic Study of Osteoporosis, Biology Methods and Protocols (2024)

5. Bone Biosensors: Knowing the Present and Predicting the Future, Journal of Micromechanics and Microengineering (2016)

6. Annual Incidence of Osteoporotic Hip Fractures in Iran: A Systematic Review and Meta-Analysis, BMC Geriatrics (2021)

7. Direct Costs of Osteoporosis-Related Hip Fractures: Protocol for a Cross-Sectional Analysis of a National Database, BMJ Open (2017)

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Bagher Larijani (5)

Farideh Razi (3)

Style	Citing Format
MLA	Khashayar P, et al.. "A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps." 3 Biotech, vol. 7, no. 5, 2017, pp. -.
APA	Khashayar P, Amoabediny G, Larijani B, Hosseini M, Verplancke R, De Keersmaecker M, Adriaens A, Goemaere S, Fiers T, Vanfleteren J (2017). A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps. 3 Biotech, 7(5), -.
Chicago	Khashayar P, Amoabediny G, Larijani B, Hosseini M, Verplancke R, De Keersmaecker M, Adriaens A, Goemaere S, Fiers T, Vanfleteren J. "A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps." 3 Biotech 7, no. 5 (2017): -.
Harvard	Khashayar P et al. (2017) 'A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps', 3 Biotech, 7(5), pp. -.
Vancouver	Khashayar P, Amoabediny G, Larijani B, Hosseini M, Verplancke R, De Keersmaecker M, et al.. A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps. 3 Biotech. 2017;7(5):-.
BibTex	@article{ author = {Khashayar P and Amoabediny G and Larijani B and Hosseini M and Verplancke R and De Keersmaecker M and Adriaens A and Goemaere S and Fiers T and Vanfleteren J}, title = {A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps}, journal = {3 Biotech}, volume = {7}, number = {5}, pages = {-}, year = {2017} }
RIS	TY - JOUR AU - Khashayar P AU - Amoabediny G AU - Larijani B AU - Hosseini M AU - Verplancke R AU - De Keersmaecker M AU - Adriaens A AU - Goemaere S AU - Fiers T AU - Vanfleteren J TI - A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps JO - 3 Biotech VL - 7 IS - 5 SP - EP - PY - 2017 ER -

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