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An Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Osteocalcin Publisher



Khashayar P1, 2, 3 ; Amoabediny G4, 5 ; Hosseini M1 ; Verplancke R2 ; Razi F2 ; Vanfleteren J6 ; Larijani B6
Authors
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Authors Affiliations
  1. 1. Nanobiotechnology Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, 14395, Iran
  2. 2. Center for Microsystems Technology, Ghent University, Gent-Zwijnaarde, 9052, Belgium
  3. 3. Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, 14114, Iran
  4. 4. Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, Tehran, 14395, Iran
  5. 5. Research Center for New Technology in Life Sciences Engineering, Nanobiotechnology Department, University of Tehran, Tehran, 14395, Iran
  6. 6. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, 14114, Iran

Source: IEEE Sensors Journal Published:2017


Abstract

This paper reports a step-wise protocol to immobilize corresponding antibody on gold electrodes for electrochemical measurement of Osteocalcin (Oc), a bone turnover marker, levels in human serum. To our knowledge this is the first such sensor developed for Oc measurement. The magnitude of electrochemical response current was linearly dependent on Oc concentration with an R square of 0.99. The detection limit was calculated to be 0.65 ng/mL. The sensor was capable to assess antigen levels of 2.5-90 ng/mL. The correlation between our sensor results and that of electrochemiluminescence (ECLIA), the current state-of-art, was also investigated and the results showed a high correlation (slope of 0.97 and R2 of 0.98) between the two systems. This is while the total assay time for the Oc sensor is about 5 min and ECLIA needs several hours to be performed. © 2017 IEEE.
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Experts (# of related papers)
Bagher Larijani (3)
Farideh Razi (1)