16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population

16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population Publisher

Rezasoltani S^{1, 7, 8} ; Azizmohammad Looha M^{2, 7} ; Asadzadeh Aghdaei H^{2, 7} ; Jasemi S^{3, 7} ; Sechi LA^{3, 7, 9} ; Gazouli M^{4, 7} ; Sadeghi A^{5, 7} ; Torkashvand S^{2, 7} ; Baniali R^{2, 7} ; Schluter H^{1, 7} ; Zali MR^{5, 7} ; Feizabadi MM^{3, 6, 7}

Show Affiliations

1. Section Mass Spectrometric Proteomics, Diagnostic Center, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, 20246, Germany
2. Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Hospital, Aachen, 52057, Germany
3. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, 19835-178, Iran
4. Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43b, Sassari, 07100, Italy
5. Struttura Complessa Microbiologia e Virologia, Azienda Ospedaliera Universitaria, Sassari, 07100, Italy
6. Department of Basic Medical Sciences, Laboratory of Biology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
7. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, 19835-178, Iran
8. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, 19835-178, Iran
9. Thoracic Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran

Source: Gut Pathogens Published:2024

Abstract

Background: Colorectal cancer (CRC) poses a significant healthcare challenge, accounting for nearly 6.1% of global cancer cases. Early detection, facilitated by population screening utilizing innovative biomarkers, is pivotal for mitigating CRC incidence. This study aims to scrutinize the fecal and salivary microbiomes of CRC-positive individuals (CPs) in comparison to CRC-negative counterparts (CNs) to enhance early CRC diagnosis through microbial biomarkers. Material and methods: A total of 80 oral and stool samples were collected from Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran, encompassing both CPs and CNs undergoing screening. Microbial profiling was conducted using 16S rRNA sequencing assays, employing the Nextera XT Index Kit on an Illumina NovaSeq platform. Results: Distinct microbial profiles were observed in saliva and stool samples of CPs, diverging significantly from those of CNs at various taxonomic levels, including phylum, family, and species. Saliva samples from CPs exhibited abundance of Calothrix parietina, Granulicatella adiacens, Rothia dentocariosa, and Rothia mucilaginosa, absent in CNs. Additionally, Lachnospiraceae and Prevotellaceae were markedly higher in CPs' feces, while the Fusobacteria phylum was significantly elevated in CPs' saliva. Conversely, the non-pathogenic bacterium Akkermansia muciniphila exhibited a significant decrease in CPs' fecal samples compared to CNs. Conclusion: Through meticulous selection of saliva and stool microbes based on Mean Decrease GINI values and employing logistic regression for saliva and support vector machine models for stool, we successfully developed a microbiota test with heightened sensitivity and specificity for early CRC detection. © The Author(s) 2024.

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Style	Citing Format
MLA	Rezasoltani S, et al.. "16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population." Gut Pathogens, vol. 16, no. 1, 2024, pp. -.
APA	Rezasoltani S, Azizmohammad Looha M, Asadzadeh Aghdaei H, Jasemi S, Sechi LA, Gazouli M, Sadeghi A, Torkashvand S, Baniali R, Schluter H, Zali MR, Feizabadi MM (2024). 16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population. Gut Pathogens, 16(1), -.
Chicago	Rezasoltani S, Azizmohammad Looha M, Asadzadeh Aghdaei H, Jasemi S, Sechi LA, Gazouli M, Sadeghi A, et al.. "16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population." Gut Pathogens 16, no. 1 (2024): -.
Harvard	Rezasoltani S et al. (2024) '16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population', Gut Pathogens, 16(1), pp. -.
Vancouver	Rezasoltani S, Azizmohammad Looha M, Asadzadeh Aghdaei H, Jasemi S, Sechi LA, Gazouli M, et al.. 16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population. Gut Pathogens. 2024;16(1):-.
BibTex	@article{ author = {Rezasoltani S and Azizmohammad Looha M and Asadzadeh Aghdaei H and Jasemi S and Sechi LA and Gazouli M and Sadeghi A and Torkashvand S and Baniali R and Schluter H and Zali MR and Feizabadi MM}, title = {16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population}, journal = {Gut Pathogens}, volume = {16}, number = {1}, pages = {-}, year = {2024} }
RIS	TY - JOUR AU - Rezasoltani S AU - Azizmohammad Looha M AU - Asadzadeh Aghdaei H AU - Jasemi S AU - Sechi LA AU - Gazouli M AU - Sadeghi A AU - Torkashvand S AU - Baniali R AU - Schluter H AU - Zali MR AU - Feizabadi MM TI - 16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population JO - Gut Pathogens VL - 16 IS - 1 SP - EP - PY - 2024 ER -

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