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Thioflavin T Effect in Diabetic Wistar Rats: Reporting the Antihyperglycemic Property of an Amyloid Probing Dye Publisher Pubmed



Najafian M1 ; Amini S2 ; Dehestani B3 ; Parivar K4 ; Ebrahimhabibi A3, 5
Authors
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Authors Affiliations
  1. 1. Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
  2. 2. Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  5. 5. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Pharmacological Reports Published:2015


Abstract

Background Thioflavin T (ThT) is a well-known probe of amyloid fibrils with a benzothiazole core structure. As a compound with partial inhibitory effect on alpha-amylase, the results of oral ThT administration were investigated on a streptozotocin (STZ)-induced rat model of diabetes. Methods STZ was administered intraperitoneally for induction of diabetes. Afterwards, doses of 2, 8, 16, and 32 mg/kg of ThT were used in diabetic and non-diabetic rats. Blood glucose levels, lipid profiles, alpha-amylase activity, food and water intake and urine volume were assessed. Docking was also performed to evaluate the inhibitory effect of ThT on alpha-amylase. Results Upon treatment with ThT, blood glucose levels and lipid profile of diabetic rats improved significantly. Furthermore, alpha-amylase serum levels of treated animals decreased compared to the control group, suggesting a possible effect of ThT on this digestive enzyme. On the other hand, the food intake of treated animals showed a decrease. ThT effects were also seen to some extent in the non-diabetic group. Conclusion ThT is suggested to be a potentially useful compound in treatment and prevention of diabetes and associated complications. © 2014 Institute of Pharmacology, Polish Academy of Sciences.