Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy

Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy Publisher Pubmed

Sharifi M¹ ; Sohrabi MJ² ; Hosseinali SH³ ; Hasan A^{4, 5} ; Kani PH⁶ ; Talaei AJ⁶ ; Karim AY^{7, 8} ; Nanakali NMQ^{9, 10} ; Salihi A^{7, 11} ; Aziz FM⁷ ; Yan B¹² ; Khan RH¹³ ; Saboury AA¹⁴ ; Falahati M¹

Show Affiliations

1. Department of Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4. Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha, 2713, Qatar
5. Biomedical Research Center, Qatar University, Doha, 2713, Qatar
6. Department of Biotechnology, Faculty of Basic Science, East Tehran Branch, Islamic Azad University, Tehran, Iran
7. Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq
8. Research Center, Knowledge University, Erbil, Kurdistan Region, Iraq
9. Department of Biology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq
10. Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq
11. Department of Medical Analysis, Faculty of Science, Tishk International University, Erbil, Iraq
12. School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China
13. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India
14. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2020

Abstract

Nanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes. However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating enzymatic activity and their medical applications. To overcome these challenges, it is necessary to explore how enzymes attach to nanomaterials and their properties are affected by such interactions. In this review we present an overview on the different strategies of the enzyme immobilization into the NPs and their corresponding stability against temperature and pH. The effects of surface charge, particle size, morphology, and aggregation of NPs on the stability of immobilized enzymes were summarized. The activity of immobilized enzyme into the NPs was reviewed to disclose more detail regarding the interaction of biomolecules with NPs. The combination of enzyme immobilization with prodrugs was also reviewed as a promising approach for biomedical application of enzyme in cancer therapy. Finally, the current challenges and future applications of NPs in enzyme immobilization and the utilization of immobilized enzyme toward prodrug activation in cytoplasm of cancer cells were presented. In conclusion, this review may pave the way for providing a perspective on development to the industrial and clinical translation of immobilized enzymes. © 2019

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Style	Citing Format
MLA	Sharifi M, et al.. "Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy." International Journal of Biological Macromolecules, vol. 143, no. , 2020, pp. 665-676.
APA	Sharifi M, Sohrabi MJ, Hosseinali SH, Hasan A, Kani PH, Talaei AJ, Karim AY, Nanakali NMQ, Salihi A, Aziz FM, Yan B, Khan RH, Saboury AA, Falahati M (2020). Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy. International Journal of Biological Macromolecules, 143(), 665-676.
Chicago	Sharifi M, Sohrabi MJ, Hosseinali SH, Hasan A, Kani PH, Talaei AJ, Karim AY, et al.. "Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy." International Journal of Biological Macromolecules 143, no. (2020): 665-676.
Harvard	Sharifi M et al. (2020) 'Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy', International Journal of Biological Macromolecules, 143(), pp. 665-676.
Vancouver	Sharifi M, Sohrabi MJ, Hosseinali SH, Hasan A, Kani PH, Talaei AJ, et al.. Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy. International Journal of Biological Macromolecules. 2020;143():665-676.
BibTex	@article{ author = {Sharifi M and Sohrabi MJ and Hosseinali SH and Hasan A and Kani PH and Talaei AJ and Karim AY and Nanakali NMQ and Salihi A and Aziz FM and Yan B and Khan RH and Saboury AA and Falahati M}, title = {Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy}, journal = {International Journal of Biological Macromolecules}, volume = {143}, number = {}, pages = {665-676}, year = {2020} }
RIS	TY - JOUR AU - Sharifi M AU - Sohrabi MJ AU - Hosseinali SH AU - Hasan A AU - Kani PH AU - Talaei AJ AU - Karim AY AU - Nanakali NMQ AU - Salihi A AU - Aziz FM AU - Yan B AU - Khan RH AU - Saboury AA AU - Falahati M TI - Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy JO - International Journal of Biological Macromolecules VL - 143 IS - SP - 665 EP - 676 PY - 2020 ER -

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