Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability

Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability Publisher

Poorakbar E^{1, 2, 3} ; Shafiee A² ; Saboury AA³ ; Rad BL¹ ; Khoshnevisan K⁴ ; Mamani L⁵ ; Derakhshankhah H⁶ ; Ganjali MR⁷ ; Hosseini M⁸

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1. Department of Biology, Faculty of Sciences, Payame Noor University, P.O.Box: 19395-3697, Tehran, Iran
2. Department of Pharmaceutics, Tehran University of Medical Science, Tehran, Iran
3. Institute of Biophysics and Biochemistry, University of Tehran, Tehran, Iran
4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
6. Pharmacutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
7. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
8. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

Source: Process Biochemistry Published:2018

Abstract

Magnetic gold mesoporous silica nanoparticle core shells (mAu@PSNs) were fabricated as a support and their size, morphology and structure was further characterized by X-Ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM), dynamic light scattering (DLS) and thermal gravimetric analysis (TGA). Cellulase (CEL) immobilization on mAu@PSNs was performed via covalent bonding. Fourier transform infrared (FTIR) spectroscopy confirmed the successful binding of enzyme to mAu@PSNs while Bradford assay determined the binding efficiency to be 76%. The enzyme activity was measured at different pHs and temperatures by FPase method using Whatman filter paper as the substrate. The immobilized enzyme maintained 58% of its initial catalytic activity after nine hours. In this research, a new nano-system was designed as a solid support for cellulase immobilization which enhanced its thermal stability and facilitated its long term storage. In addition, the immobilized enzyme can be applied in a broader temperature and pH ranges while enzyme separation can be simply carried out by an external magnet. © 2018 Elsevier Ltd

2. Recent Advances of Cellulase Immobilization Onto Magnetic Nanoparticles: An Update Review, Magnetochemistry (2019)

3. Immobilization of L-Asparaginase on Aspartic Acid Functionalized Graphene Oxide Nanosheet: Enzyme Kinetics and Stability Studies, Chemical Engineering Journal (2018)

4. Biomolecular Corona Dictates Aβ Fibrillation Process, ACS Chemical Neuroscience (2018)

5. Solid Silica Nanoparticles: Applications in Molecular Imaging, Contrast Media and Molecular Imaging (2015)

6. Smart Mesoporous Silica Nanoparticles for Controlled-Release Drug Delivery, Nanotechnology Reviews (2016)

Experts (# of related papers)

Fatemeh Atyabi (1)

Rassoul Dinarvand (1)

Style	Citing Format
MLA	Poorakbar E, et al.. "Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability." Process Biochemistry, vol. 71, no. , 2018, pp. 92-100.
APA	Poorakbar E, Shafiee A, Saboury AA, Rad BL, Khoshnevisan K, Mamani L, Derakhshankhah H, Ganjali MR, Hosseini M (2018). Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability. Process Biochemistry, 71(), 92-100.
Chicago	Poorakbar E, Shafiee A, Saboury AA, Rad BL, Khoshnevisan K, Mamani L, Derakhshankhah H, Ganjali MR, Hosseini M. "Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability." Process Biochemistry 71, no. (2018): 92-100.
Harvard	Poorakbar E et al. (2018) 'Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability', Process Biochemistry, 71(), pp. 92-100.
Vancouver	Poorakbar E, Shafiee A, Saboury AA, Rad BL, Khoshnevisan K, Mamani L, et al.. Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability. Process Biochemistry. 2018;71():92-100.
BibTex	@article{ author = {Poorakbar E and Shafiee A and Saboury AA and Rad BL and Khoshnevisan K and Mamani L and Derakhshankhah H and Ganjali MR and Hosseini M}, title = {Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability}, journal = {Process Biochemistry}, volume = {71}, number = {}, pages = {92-100}, year = {2018} }
RIS	TY - JOUR AU - Poorakbar E AU - Shafiee A AU - Saboury AA AU - Rad BL AU - Khoshnevisan K AU - Mamani L AU - Derakhshankhah H AU - Ganjali MR AU - Hosseini M TI - Synthesis of Magnetic Gold Mesoporous Silica Nanoparticles Core Shell for Cellulase Enzyme Immobilization: Improvement of Enzymatic Activity and Thermal Stability JO - Process Biochemistry VL - 71 IS - SP - 92 EP - 100 PY - 2018 ER -

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