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Analysis of Resistance to Macrolide-Lincosamide-Streptogramin B Among Meca-Positive Staphylococcus Aureus Isolates Publisher



Khodabandeh M1 ; Mohammadi M2 ; Abdolsalehi MR1 ; Alvandimanesh A3 ; Gholami M4 ; Bibalan MH5 ; Pournajaf A6 ; Kafshgari R7 ; Rajabnia R8
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Authors Affiliations
  1. 1. Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
  3. 3. Department of Pathology, Shafa Hospital, Qazvin University of Medical Sciences, Qazvin, Iran
  4. 4. Department of Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  5. 5. Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  6. 6. Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
  7. 7. Student Research Committee, Babol University of Medical Sciences, Babol, Iran
  8. 8. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, IR, Iran

Source: Osong Public Health and Research Perspectives Published:2019


Abstract

Objectives: Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLSB) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLSB resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples. Methods: A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLSB-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates. Results: The frequency of constitutive resistance MLSB, inducible resistance MLSB and MSB resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLSB phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLSB phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively. Conclusion: Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided. © 2019 Korea Centers for Disease Control and Prevention. This is an open access article under the CC BY-NC-ND license
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