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Optimization of Solid Phase Microextraction Procedure for Determination of Paraquat Using Reduction Process Publisher



Ghavidel F1 ; Shahtaheri SJ2 ; Torabbeigi M3 ; Froushani AR4
Authors
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Authors Affiliations
  1. 1. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155-6446, Iran
  2. 2. Department of Occupational Health Engineering, School of Public Health, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, 14155-6446, Iran
  3. 3. Health, Safety and Environment School, Shahid Beheshti University of Medical Sciences, P.O. Box 16858-116, Tehran, Iran
  4. 4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155-6446, Iran

Source: Journal of Analytical Chemistry Published:2016


Abstract

Headspace solid phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry was developed for the determination of paraquat in urine. The volatile product resulting from reduction of paraquat by sodium borohydride–nickel chloride was used for HS-SPME. The calibration curve was linear from 10–1000 ng/mL and the limit of detection was 0.1 ng/mL. The optimized methods were validated using 500 and 750 ng/mL concentrations of paraquat in the spiked urine samples. The recoveries obtained in this study were 98.2% for intra-day (n = 6) and 99.2% for inter-day (n = 6), respectively, with RSD lower than 1.1%. © 2016, Pleiades Publishing, Ltd.