Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate Publisher

Samini L¹ ; Khoubnasabjafari M² ; Alimorad MM¹ ; Jouybangharamaleki V³ ; Chan HK⁴ ; Jouyban A^{5, 6}

Show Affiliations

1. Research and Development Department, Jaber Ebne Hayyan Pharmaceutical Co., Tehran, Iran
2. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4. School of Pharmacy, Faculty of Medicine and Health, University of Sydney, Sydney, 2006, NSW, Australia
5. Pharmaceutical Analysis Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
6. Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Current Analytical Chemistry Published:2020

Abstract

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 µL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 µg·L-1 in EBC. Inter-and intra-day preci-sions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method. © 2020 Bentham Science Publishers.

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Style	Citing Format
MLA	Samini L, et al.. "Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate." Current Analytical Chemistry, vol. 16, no. 8, 2020, pp. 1032-1040.
APA	Samini L, Khoubnasabjafari M, Alimorad MM, Jouybangharamaleki V, Chan HK, Jouyban A (2020). Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate. Current Analytical Chemistry, 16(8), 1032-1040.
Chicago	Samini L, Khoubnasabjafari M, Alimorad MM, Jouybangharamaleki V, Chan HK, Jouyban A. "Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate." Current Analytical Chemistry 16, no. 8 (2020): 1032-1040.
Harvard	Samini L et al. (2020) 'Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate', Current Analytical Chemistry, 16(8), pp. 1032-1040.
Vancouver	Samini L, Khoubnasabjafari M, Alimorad MM, Jouybangharamaleki V, Chan HK, Jouyban A. Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate. Current Analytical Chemistry. 2020;16(8):1032-1040.
BibTex	@article{ author = {Samini L and Khoubnasabjafari M and Alimorad MM and Jouybangharamaleki V and Chan HK and Jouyban A}, title = {Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate}, journal = {Current Analytical Chemistry}, volume = {16}, number = {8}, pages = {1032-1040}, year = {2020} }
RIS	TY - JOUR AU - Samini L AU - Khoubnasabjafari M AU - Alimorad MM AU - Jouybangharamaleki V AU - Chan HK AU - Jouyban A TI - Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate JO - Current Analytical Chemistry VL - 16 IS - 8 SP - 1032 EP - 1040 PY - 2020 ER -

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