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Co-Culture of Human Cryopreserved Fragmented Ovarian Tissue With Theca Progenitor Cells Derived From Theca Stem Cells Publisher Pubmed



Dalman A1 ; Adib S2 ; Amorim CA3 ; Pirjani R4 ; Totonchi M5 ; Valojerdi MR6
Authors
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Authors Affiliations
  1. 1. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Banihashem Avenue, Resalat Highway, PO Box 19395- 4644, Tehran, Iran
  2. 2. Faculty of Medicine, Department of Anatomical Sciences & Cognitive Neuroscience, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  3. 3. Pole de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Experimentale Et Clinique, Universite Catholique de Louvain, Avenue Hippocrate 55, Bte. B1.55.03, Bruxelles, 1200, Belgium
  4. 4. Department of Obstetrics and Gynecology, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  6. 6. Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Jalal-Ale-Ahmad Street, P.O.Box:14115–111, Tehran, Iran

Source: Journal of Assisted Reproduction and Genetics Published:2023


Abstract

Purpose: Despite the significant advances in the in vitro development of human primordial follicles, it is still a challenging approach with great potential for improvements. Therefore, the present study aimed to investigate the effect of a feeder layer of human theca progenitor cells (hTPCs) on the development of primordial follicles embedded in human ovarian tissue. Methods: Fragments of frozen-thawed ovarian tissue were activated using the vanadate-derivative dipotassium bisperoxo (5-hydroxy-pyridine-2-carboxylic) oxovanadate (V) and kit ligand for 24 h. Then, the specimens were divided into the co-culture and mono-culture groups and were cultured with and without a hTPC feeder layer for 6 days, respectively. Afterward, the follicles were counted and classified, and the hormone levels and expression levels of apoptosis- and folliculogenesis-related genes were assessed. Results: Both culture groups showed significant follicle growth (P < 0.05). However, the co-culture group had a significantly higher number of growing follicles compared to the other group (P < 0.05). Moreover, the expression levels of ZP1, ZP2, ZP3, BMP-7, AMH, and GDF9 were significantly higher in the co-culture group compared to the other group (P < 0.05), while the expression levels of P53 and CASP3 were significantly lower (P < 0.05). Also, the concentrations of estradiol, progesterone, testosterone, and androstenedione were significantly higher in the co-culture group compared to the other group (P < 0.05). Conclusion: The present study results provided novel evidence on the direct role of hTPCs in the growth and development of human primordial follicles. However, there is a need for future studies to illustrate the underlying mechanisms. Graphical abstract: [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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