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Evaluating the Mechanism Underlying Antitumor Effect of Interleukin 27 on B Cells of Chronic Lymphocytic Leukemia Patients Publisher Pubmed



Manouchehridoulabi E1, 2, 3 ; Abbaspour S1, 2 ; Rostami S4 ; Faranoush M5 ; Ghahramanfard F1, 2 ; Pak F1 ; Barati M6, 7 ; Kokhaei P1, 2, 8 ; Momtaziborojeni AA9, 10
Authors
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Authors Affiliations
  1. 1. Faculty of Medicine, Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
  2. 2. Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran
  3. 3. Students Research Committee, Semnan University of Medical Sciences, Semnan, Iran
  4. 4. Hematology-Oncology and Stem Cell Transplantation, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Iran University of Medical Sciences, Rasool Akram Complex Medical Center, MAHAK Hospital, Tehran, Iran
  6. 6. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
  7. 7. Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  8. 8. Department of Oncology, Cancer Centre Karolinska, Karolinska University, Hospital Solna, Stockholm, Sweden
  9. 9. Halal Research Center of IRI, FDA, Tehran, Iran
  10. 10. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Journal of Cellular Physiology Published:2020


Abstract

Chronic lymphocyte leukemia (CLL) is a B-cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL-27) can reduce B-cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL-27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL-27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL-27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL-27 and IL-27 receptor were determined using flow cytometry and real-time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL-27 on B cells of patients with CLL, Annexin V-FITC and 7-AAD (BioLegend) fluorescent dyes were used. In addition, the IL-27 effect on activation of T cell and NK cell was determined by determining CD96 molecule expression. IL-27 and IL-27 receptor expression in patients with CLL was significantly lower than that of normal subjects (p '.05). IL-27 enhanced apoptosis of B cells in patients with CLL (p '.05) but this effect was not significantly observed in B cells of normal subjects (p '.05). Consequently, IL-27 reduced the proliferation of B cells and enhanced NK cell activity (p '.05). IL-27, through inducing apoptosis, can exert an inhibitory effect on cancer B cells of CLL patients with minimal effect on normal B cells. © 2020 Wiley Periodicals LLC
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