Determination of Trace Amounts of Risperidone in Human Urine Sample by Hollow Fiber Liquid-Phase Microextraction Combined With High Performance Liquid Chromatography Publisher



Charmahali G¹ ; Chaharmahali M² ; Tafti FF³
Source: Biosciences Biotechnology Research Asia Published:2015

Abstract

Risperidone is an antipsychotic drug mainly used to treat schizophrenia. In this paper, for the first time a three-phase hollow uber liquid-phase microextraction (HF-LPME) method combined with high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was presented to detection and quantification of risperidone in urine samples. Different factors affecting the HF-LPME extraction recovery were studied and optimized. Under optimal extraction condition, risperidone could be determined within the linearity range with a good correlation coefficient (r2 > 0.9980). The limit of detection for risperidone was 0.2 ng mL-1, and the intra- and inter-day relative standard deviations were no more than 1.2% and 3.2%, respectively. This procedure afforded a simple, sensitive and inexpensive method with a high extraction efficiency for determination of risperidone. Finally, it was applied to determination and quantification of risperidone in human urine samples.