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Dispersive Liquid–Liquid Microextraction for the Determination of Salivary Melatonin As a Biomarker of Circadian Rhythm Publisher



Pourhossein M1, 2 ; Shahtaheri SJ1, 2, 3 ; Mazloumi A1, 2 ; Rahimiforoushani A4 ; Helmikohneshahri M1, 2 ; Khani HM5
Authors
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Authors Affiliations
  1. 1. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, 141556445, Iran
  2. 2. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, 141556445, Iran
  3. 3. Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, 141556445, Iran
  4. 4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, 141556445, Iran
  5. 5. Department of Occupational Health Engineering, School of Public Health, Mazandaran University of Medical Sciences, Sari, 549156851, Iran

Source: Journal of Analytical Chemistry Published:2018


Abstract

Abstract: Melatonin (N-acetyl-methoxytryptamine) is secreted from a part of the brain called suprachiasmatic nuclei. The biological clock of the body or the circadian rhythm is regulated through the secretion of hormones such as melatonin and cortisol. The dispersive liquid–liquid microextraction (DLLME) was optimized for the determination of human salivary melatonin. Different variables including the nature of extracting and dispersing solvent, their volumes, sample pH, ionic strength, extraction and centrifugation times were screened using One Factor At a Time design and then, the significant variables were selected as optimum values. Accuracy and precision of the optimized method were evaluated at concentrations of 50, 100, 250 pg/mL by achieving CV% for day-to-day and within-day reproducibility. Considering the appropriate results of the study, DLLME procedure can be used for the determination of melatonin hormone in saliva samples. © 2018, Pleiades Publishing, Ltd.