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Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia Coli O25b/St131 Isolates From Patients With Urinary Tract Infection in Iran Publisher Pubmed



Rasoulinasab M1 ; Shahcheraghi F1 ; Feizabadi MM2 ; Nikmanesh B3 ; Hajihasani A1 ; Sabeti S4 ; Aslani MM1
Authors
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Authors Affiliations
  1. 1. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Department of Microbiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran
  3. 3. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Microbial Drug Resistance Published:2021


Abstract

Escherichia coli serogroup O25b-sequence type 131 (E. coli O25b/ST131) is known as a multidrug-resistant organism with high virulence potential and has received attention internationally. We aim to investigate the prevalence of O25b/ST131 and the distribution of blaCTX-M-15, pathogenicity island (PAI) markers, phylogenetic groups, and H-antigen typing in the E. coli O25b/ST131 isolated from patients with urinary tract infection (UTI) in Tehran, the capital of Iran. Seventy (26.9%) E. coli isolates were identified as O25b/ST131. There was also a significant difference in the prevalence of virulence genes, including papA, sfa, sat, cnf1, iutA, kpMII, traT, and usp, in the O25b/ST131 isolates rather than non-O25b/ST131 ones (p ≤ 0.05). Furthermore, 78% of the O25b/ST131 isolates carried four to seven PAIs, while 71% of non-O25b/ST131 isolates carried two to four PAI markers (p ≤ 0.05). Our study showed that in addition to H4, other H-antigens may play a role in the O25b/ST131 virulence potential. Besides, a significant association was found between the history of previous UTIs and infection among the O25b/ST131 clone isolates. Pulsed-field gel electrophoresis revealed circulating of O25b:H4-ST131/PST43 clone in both hospital and community. Approximately one in every three uropathogenic E. coli isolates was the O25b/ST131 clone, representing a significant public health threat. Practical investigation on O25b/ST131 can be helpful in better understanding of ST131 evolution and controlling UTI in hospitals. © Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.
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