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Investigating the Impact of Collagen-Chitosan Derived From Scomberomorus Guttatus and Shrimp Skin on Second-Degree Burn in Rats Model Publisher



Fatemi MJ2, 8 ; Garahgheshlagh SN1, 2, 8 ; Ghadimi T2, 8 ; Jamili S1, 5 ; Nourani MR4 ; Sharifi AM3 ; Saberi M6 ; Amini N7, 9 ; Sarmadi VH7, 9 ; Yazdiamirkhiz SY10
Authors
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Authors Affiliations
  1. 1. Department of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Pharmacology and Razi Drug Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Division of Genomics, Baqiyatallah University of Medical Sciences, Iran
  5. 5. Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
  6. 6. Medicine, Quran and Hadith Research Center & Department of Community Medicine, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  7. 7. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Plastic and Reconstructive Surgery, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Tehran, Iran
  9. 9. Institutes of Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Iran
  10. 10. Department of Foreign Languages, Tehran University of Medical Sciences, Iran

Source: Regenerative Therapy Published:2021


Abstract

Background: The present study focused on burning as one of the main causes of mortality with detrimental economic and social effects in the world. The purpose of this study was to investigate the impact of collagen-chitosan gel extracted from Scomberomorus guttatus and shrimp skin in the treatment of second degree burn healing among rats. Materials & method: To fulfill the purpose of the study, chitosan and collagen were extracted respectively from shrimp and Scomberomorus guttatus skin waste by the acid-based method and were evaluated by using Pico Tag, SDS-PAGE. The burn wound healing efficiency of marine collagen-chitosan gel was examined in vivo using rats. Three different ratios of collagen and chitosan blend (Col-CH, 1:3, 1:1 and 3:1) were prepared to obtain the most effective Col-CH gel for burn wound healing and were compared to the animals treated with silver sulfadiazine ointment. Healing burn wound was studied by measuring wound surface area with Image J and histopathologic examination was carried out based on the mean of epithelialization, fibroblastic cells, acute and chronic inflammatory cells, angiogenesis, structure collagen and the amount of collagen on days 15 and 25 post-burn. Results: The results of SDS-PAGE indicated that the extracted collagen was type I and it was composed of two α (α1 and α2) chains. Amino acid analysis showed a much higher glaycin content in extracted collagen which amounted to one-third of the total amino. The wound surface measurement showed a significant reduction in wound size in the group treated with Col-CH (3:1) compared to silver-sulfadiazine treated group on 15th and 25th days. Histopathological findings represented a high score in epithelialization, collagen, collagen structure, fibroblast cell and a decrease in inflammatory cells infiltration in Col-CH (3:1) treated group on 25th day. The most obvious finding of the present study is that chitosan-collagen gel (3:1) represented a better efficacy compared to sulfadiazine in burn wound healing on day 25 post-burn. © 2021 The Japanese Society for Regenerative Medicine
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