Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Encapsulation of Mentha Aquatica Methanol Extract in Alginate Hydrogel Promotes Wound Healing in a Murine Model of Pseudomonas Aeruginosa Burn Infection Publisher



Solhtalab E1 ; Nikokar I1, 2 ; Mojtahedi A3 ; Shokri R4 ; Karimian P5 ; Mahdavi E2 ; Faezi S1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  2. 2. Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
  3. 3. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Pathology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

Source: International Journal of Biological Macromolecules Published:2024


Abstract

Burn injuries are the fourth most prevalent devastating form of trauma worldwide. Among the most extensively explored materials, composite dressings with alginate loaded with herbal extract can be mentioned. This research aimed to develop a sodium alginate (SA)-based hydrogel encapsulated with Mentha aquatica (MA) methanol extract and investigate its therapeutic efficacy in the infected burn mouse model. SEM, FTIR, in vitro extract release, HPLC, mechanical test, contact angle, swelling, degradability, and temperature response properties were used to analyze the hydrogel scaffold's physicochemical structure. Additionally, the antibacterial activity and MIC level of the extract, cell cytotoxicity, and macroscopic and microscopic analysis of the wound healing process were done using Masson's trichrome and hematoxylin-eosin staining. The physicochemical properties of the SA hydrogel encapsulated with MA extract were verified. The lowest inhibitory dose of the extract was determined to be 12.5 mg/ml. Application of SA/MA hydrogel to localized wounds of deep third-degree burns demonstrated faster tissue regeneration, collagen recovery, and eradication of bacterial infection. This research focused on the design and the preparation of a novel and effective biomaterials-based medical product, which has the potential to rehabilitate infected and injured skin tissue; therefore, it can be a promising candidate for wound dressing applications. © 2024 Elsevier B.V.