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The Effect of Combined Pulsed Wave Low-Level Laser Therapy and Mesenchymal Stem Cell-Conditioned Medium on the Healing of an Infected Wound With Methicillin-Resistant Staphylococcal Aureus in Diabetic Rats Publisher Pubmed



Kouhkheil R1 ; Fridoni M1 ; Piryaei A2, 3 ; Taheri S4 ; Chirani AS4 ; Anarkooli IJ1 ; Nejatbakhsh R1 ; Shafikhani S5, 6 ; Schuger LA7 ; Reddy VB8 ; Ghoreishi SK9 ; Jalalifirouzkouhi R10 ; Chien S11, 12 ; Bayat M13
Authors
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Authors Affiliations
  1. 1. Department of Biology and Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  2. 2. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Medicine, Division of Hematology/Oncology, Rush University Medical Center, Chicago, IL, United States
  6. 6. Department of Immunology/Microbiology, Cancer Center, Chicago, IL, United States
  7. 7. Department of Pathology, The University of Chicago, Chicago, IL, United States
  8. 8. Pathology Department, Rush University Medical Center, Chicago, IL, United States
  9. 9. Department of Statistics, University of Qom, Qom, Iran
  10. 10. School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  11. 11. Noveratech LLC of Louisville, Louisville, KY, United States
  12. 12. Department of Surgery, University of Louisville, Louisville, KY, United States
  13. 13. Cellular and Molecular Biology Research Center, and Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Cellular Biochemistry Published:2018


Abstract

This study aims to investigate the combined effects of Pulsed wave low-level laser therapy (PW LLLT) and human bone marrow mesenchymal stem cell-conditioned medium (hBM-MSC-CM) on the microbial flora and tensiometrical properties of an infected wound model with methicillin-resistant staphylococcal aureus (MRSA) in an experimental model for Type 1 diabetes mellitus (TIDM). TIDM was induced in rats by streptozotocin (STZ). One full-thickness excision was made on the backs of the rats. Next, the rats were divided into the following groups: Group 1 was the control (placebo) group; Group 2 received hBM-MSCs-CM four times; Group 3 were laser PWLLLT (890 nm, 80 Hz, 0.2 J/cm 2 ); and Group 4 received hBM-MSCs-CM +LASER. Wounds were infected with MRSA. Microbiological examinations were performed on days 4, 7, and 15. Tensiometerical examinations were carried out on the 15th day. One-way analysis of variance showed that laser and CM alone and/or in combination significantly increases the tensiomerical properties of the repaired wounds compared with control wounds. A combination of PW laser and CM was statistically more effective than other treated groups. Two-way analysis of variance showed that laser and CM alone and/or in combination significantly decreases the colony-forming units (CFUs) compared with the control group. The application of hBM-MSC-CM and PWlaser alone and/or together significantly accelerates the wound-healing process in MRSA-infected cutaneous wounds in TI DM in rats. Additionally, a combined application of hBM-MSC-CM and PWlaser demonstrates a synergistic effect on the wound-healing process in MRSA-infected cutaneous wounds in Type I DM rats. © 2018 Wiley Periodicals, Inc.
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