Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases

Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases Publisher Pubmed

Arastehfar A¹ ; Fang W^{2, 3} ; Alhatmi AMS^{1, 4} ; Afsarian MH⁵ ; Daneshnia F¹ ; Bakhtiari M⁶ ; Sadati SK⁶ ; Badali H⁷ ; Khodavaisy S⁸ ; Hagen F¹ ; Liao W^{2, 3} ; Pan W^{2, 3} ; Zomorodian K⁶ ; Boekhout T^{1, 2, 9}

Show Affiliations

1. Westerdijk Fungal Biodiversity Institute, Utrecht, Netherlands
2. Department of Dermatology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China
3. Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China
4. Ministry of Health, Directorate General of Health Services, Ibri, Oman
5. Departments of Parasitology and Mycology, Fasa University of Medical Sciences, Fasa, Iran
6. Basic Sciences in Infectious Diseases Research Center, Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
7. Department of Medical Mycology/Invasive Fungi Research Center (IFRC), School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
8. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
9. Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands

Source: Medical Mycology Published:2019

Abstract

Although Cyberlindnera fabinaii is a rare opportunist yeast species, its ability to cause septicemia, produce biofilm, and rapid acquisition of resistance to fluconazole and voriconazole, reinforced the urge for its identification from its closely related species. Widely used biochemical assays mainly identify Cyberlindnera fabinaii as Cyberlindnera jadinii and Wickerhamomyces anomalus, resulting in underestimation of this yeast in clinical settings. Moreover, the urge for a reliable molecular means of identification remains unsolved for 28 years. In order to unequivocally differentiate Cy. fabianii, Cy. mississipiensis, Cy. jadinii, and W. anomalus, we designed a dual-function multiplex polymerase chain reaction (PCR) assay. Challenging our dual-function multiplex PCR assay with 30 most clinically important yeast species, proved its specificity. Although conventional PCR could differentiate four target species, the real-time PCR counterpart due to Tm overlap misidentified Cy. mississipiensis as Cy. jadinii. Alongside of presenting a comprehensive literature review of published cases of Cy. fabianii from 1990 to 2018, we collected various clinical isolates from Tehran, Shiraz, and Fasa (July 1, 2017, to December 31, 2017) to find a passive relative distribution of these closely-related species in Iran. Subjecting our Iranian collection of yeast isolates to matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) MS and LSU and ITS rDNA sequencing revealed six isolates of Cy. fabianii (central venous catheter n = 2 and vaginal swabs n = 4) and one isolate of Cy. jadinii (vaginal swabs). Due to the use of biochemical assays in global ARTEMIS study, we encourage reidentification of clinical isolates of Cy. jadinii and Cy. jadinii using MALDI-TOF or Sanger sequencing that might lead to correcting the distribution of this fungus. © The Author(s) 2019.

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Style	Citing Format
MLA	Arastehfar A, et al.. "Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases." Medical Mycology, vol. 57, no. 7, 2019, pp. 833-840.
APA	Arastehfar A, Fang W, Alhatmi AMS, Afsarian MH, Daneshnia F, Bakhtiari M, Sadati SK, Badali H, Khodavaisy S, Hagen F, Liao W, Pan W, Zomorodian K, Boekhout T (2019). Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases. Medical Mycology, 57(7), 833-840.
Chicago	Arastehfar A, Fang W, Alhatmi AMS, Afsarian MH, Daneshnia F, Bakhtiari M, Sadati SK, et al.. "Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases." Medical Mycology 57, no. 7 (2019): 833-840.
Harvard	Arastehfar A et al. (2019) 'Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases', Medical Mycology, 57(7), pp. 833-840.
Vancouver	Arastehfar A, Fang W, Alhatmi AMS, Afsarian MH, Daneshnia F, Bakhtiari M, et al.. Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases. Medical Mycology. 2019;57(7):833-840.
BibTex	@article{ author = {Arastehfar A and Fang W and Alhatmi AMS and Afsarian MH and Daneshnia F and Bakhtiari M and Sadati SK and Badali H and Khodavaisy S and Hagen F and Liao W and Pan W and Zomorodian K and Boekhout T}, title = {Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases}, journal = {Medical Mycology}, volume = {57}, number = {7}, pages = {833-840}, year = {2019} }
RIS	TY - JOUR AU - Arastehfar A AU - Fang W AU - Alhatmi AMS AU - Afsarian MH AU - Daneshnia F AU - Bakhtiari M AU - Sadati SK AU - Badali H AU - Khodavaisy S AU - Hagen F AU - Liao W AU - Pan W AU - Zomorodian K AU - Boekhout T TI - Unequivocal Identification of an Underestimated Opportunistic Yeast Species, Cyberlindnera Fabianii, and Its Close Relatives Using a Dual-Function Pcr and Literature Review of Published Cases JO - Medical Mycology VL - 57 IS - 7 SP - 833 EP - 840 PY - 2019 ER -

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