Combined Chemo-Magnetic Field-Photothermal Breast Cancer Therapy Based on Porous Magnetite Nanospheres Publisher Pubmed



Sharifi M^{1, 2} ; Hasan A^{3, 4} ; Nanakali NMQ^{5, 6} ; Salihi A⁷ ; Qadir FA⁷ ; Muhammad HA⁸ ; Shekha MS⁷ ; Aziz FM⁷ ; Amen KM^{9, 10} ; Najafi F¹¹ ; Yousefimanesh H¹² ; Falahati M¹ Show Affiliations
Source: Scientific Reports Published:2020

Abstract

The efficacy of different modalities of treating breast cancer is inhibited by several limitations such as off-targeted drug distribution, rapid drug clearance, and drug resistance. To overcome these limitations, we developed Lf-Doxo-PMNSs for combined chemo-MF-PTT. The PMNSs were synthesized by hydrothermal method and their physicochemical properties were examined by FE-SEM, TEM, DLS, TGA, XRD investigations. The cytotoxicity of as-synthesized NPs against 4T1 cells was carried out by MTT and flow cytometry assays. Afterwards, the anti-cancer activities of as-synthesized Lf-Doxo-PMNSs on the tumor status, drug distribution and apoptosis mechanism were evaluated. The anti-cancer assays showed that Lf-Doxo-PMNSs significantly suppressed the cancer cell proliferation and tumor weight by prolonging drug availability and potential drug loading in tumor cells; whereas they showed a minimum cytotoxicity against non-cancerous cells. Likewise, combined chemo-MF-PTT using Lf-Doxo-PMNSs displayed the highest anti-cancer activity followed by combined chemo-PTT and combined chemo-MF therapy based on altering the apoptosis mechanism. Therefore, these results showed that combined chemo-MF-PTT based on Lf-Doxo-PMNSs can be used as a promising therapeutic platform with potential targeted drug delivery and high loading capacity features as well as reducing cancer drug resistance. © 2020, The Author(s).