High-Resolution Melting Analysis in Comparison With Microscopic Method: An Experimental Study to Diagnosis of Plasmodium Species Infections in Human



Allahmoradi M¹ ; Motevalli Haghi A¹ ; Nateghpour M^{1, 2} ; Mohebali M^{1, 2} ; Raeisi A¹ ; Hosseini Safa A¹ ; Mohtasebi S¹ ; Abbaszadeh Afshar MJ¹ Show Affiliations
Source: Iranian Journal of Parasitology Published:2020

Abstract

Background: Among the human parasitic diseases, malaria is the main cause of morbidity and mortality. To prevent the high mortality and track-ing malaria elimination efforts, a prompt and sensitive diagnosis is essen-tial. This study aimed to compare High-Resolution Melting (HRM) and microscopic methods to diagnose Plasmodium falciparum and P. vivax. Methods: Eighty-one blood samples were collected from patients with clinical symptoms who were suspect to malaria in Chabahar district, southeastern Iran and also, from those who were referred to Malaria National Laboratory in the Tehran University of Medical Sciences, Tehran, Iran. Microscopic examination and HRM method were used to the diagnosis of Plasmodium parasites simultaneously. Results: Microscopic results revealed 45 positive cases (12 P. falciparum and 33 P. vivax) out of 81 collected samples while according to HRM analysis results 11 and 33 samples were identified as P. falciparum and P. vivax, respectively. HRM analysis also revealed 1 mixed infection of P. falciparum and P. malariae. Conclusion: HRM analysis provides a promising mean for simultaneous detection and discrimination of the Plasmodium spp. especially in mixed infection cases. © 2020, Tehran University of Medical Sciences (TUMS). All rights reserved.