Application of Immuno-Pcr Assay for the Detection of Serum Ige Specific to Bermuda Allergen Publisher Pubmed



Rahmatpour S^{1, 9} ; Khan AH² ; Nasiri Kalmarzi R³ ; Rajabibazl M^{4, 5} ; Tavoosidana G⁶ ; Motevaseli E⁶ ; Zarghami N^{1, 7, 8} ; Sadroddiny E⁹ Show Affiliations
Source: Molecular and Cellular Probes Published:2017

Abstract

In vivo and in vitro tests are the two major ways of identifying the triggering allergens in sensitized individuals with allergic symptoms. Both methods are equally significant in terms of sensitivity and specificity. However, in certain circumstances, in vitro methods are highly preferred because they circumvent the use of sensitizing drugs in patients. In current study, we described a highly sensitive immuno-PCR (iPCR) assay for serum IgE specific to Bermuda allergens. Using oligonucleotide-labelled antibody, we used iPCR for the sensitive detection of serum IgE. The nucleotide sequence was amplified using conventional PCR and the bands were visualized on 2.5% agarose gel. Results demonstrated a 100-fold enhancement in sensitivity of iPCR over commercially available enzyme-linked immunosorbent assay (ELISA) kit. Our iPCR method was highly sensitive for Bermuda-specific serum IgE and could be beneficial in allergy clinics. © 2016 Elsevier Ltd