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Molecular Profile of Specific Ige to Allergenic Components in Allergic Adults Using Allergen Nano-Bead Array Publisher Pubmed



Shokouhi Shoormasti R1 ; Rafaiani C2 ; Fazlollahi MR1 ; Kazemnejad A3 ; Mahloojirad M1 ; Moslemi M4 ; Abbasi J5 ; Moin M1 ; Pourpak Z1 ; Mari A2
Authors
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Authors Affiliations
  1. 1. Immunology, Asthma, and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Associated Centers for Molecular Allergology, CAAM, Rome, Italy
  3. 3. Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Blood Transfusion Organization Research Center, Tehran, Iran
  5. 5. Asthma and Allergy Center, Tehran Medical Sciences Branch of Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

Source: Clinical and Experimental Allergy Published:2022


Abstract

Background: An increasing interest in the field of molecular diagnosis of allergy has been developed in recent years and it goes to be as the routine in vitro protocol in allergy diagnosis. friendly allergen nano-bead array (FABER) is a new multiplex assay for the evaluation of specific IgE against 244 allergens including whole extracts and allergenic molecules. The research intended to assess the pattern of IgE sensitization to allergenic components of allergens in allergic adults using FABER 244. Methods: Sixty patients with allergic diseases entered this cross-sectional study. Specific IgE to 122 whole allergens extracts and 122 allergenic components were assessed using an allergen nano-bead array (FABER) for all patients. This test includes inhalant and food allergens. Results: Thirty-seven patients were male (61.7%). The mean (SD) age of patients was 30.73(±6.87) years. As the allergen nano-bead array results showed, Lolium perenne (63.3%), Phleum pratense (60%) and Platanus acerifolia (51.7%) were considered as the most common IgE sensitizations to the aeroallergen extracts. Moreover, Lol p 1, Phl p 1.0102 and Cup a 1 were found as the most frequent allergenic components in our allergic patients. Among protein families, CCD-bearing proteins, expansin, cysteine protease and profilin families illustrated the highest allergic sensitization. Conclusions: The results of the present study demonstrated that despite the higher prevalence of sensitization to Salsola kali (47.2%) using extract-based assays in the previous phase of this research, allergenic components of grasses (Lol p 1, Phl p 1.0102), Cup a 1 as well as Sal k1 as the major components of Cupressuss arizonica and Salsola kali showed the higher sensitization, respectively, in adults’ allergic patients using FABER test. © 2022 John Wiley & Sons Ltd.