Ameliorative Effects of Standardized Extract of Tamarix Stricta Boiss. on Acetic Acid-Induced Colitis Via Modulating Nitrergic Pathways Publisher



Ghanaatian N¹ ; Momtaz S^{2, 3, 4, 5} ; Bahramsoltani R^{6, 7} ; Baeeri M³ ; Abdolghaffari AH^{1, 2, 3, 4, 5} ; Rahimi R^{6, 8} Show Affiliations
Source: Biologia Published:2022

Abstract

Inflammatory bowel disease (IBD) is a chronic recurrent disease with no certain treatment. Tamarisk is a medicinal plant mentioned in Persian medicine as an effective treatment for gastrointestinal bleeding. Current study aimed to investigate the ameliorative effect of Tamarix stricta Boiss. on acetic acid-induced colitis in rats and the role of nitrergic system. The hydro-ethanolic extract was analyzed via liquid chromatography–mass spectrometry. Gallic acid and quercetin were quantified with high-performance liquid chromatography. Following induction of colitis with acetic acid, T. stricta extract (50, 100, 150, and 200 mg/kg) was orally administered for two successive days. The involvement of nitrergic system was examined, using L-NG-Nitro Arginine Methyl Ester (L-NAME) and aminoguanidine as nitric oxide synthase (NOS) inhibitors. Finally, macroscopic and microscopic analyses were performed. Tissue concentrations of inflammatory mediators including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO) were assessed. T. stricta, especially at 200 mg/kg dose, could significantly improve colitis and downregulate the inflammatory factors including TNF-α, IL-1β, and MPO (P <0.001). Concomitant use of NOS inhibitors with 200 mg/kg of the plant extract reversed the observed positive effects, which demonstrates the protective role of NO in IBD pathogenesis as a probable mechanism for T. stricta in healing process of colitis. The positive effect of T. stricta on colitis might be attributed to anti-inflammatory properties of its polyphenolic components, mainly through modulation of the nitrergic system. © 2022, Institute of Molecular Biology, Slovak Academy of Sciences.