Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency

Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency Publisher Pubmed

Bagheri Y^{1, 2} ; Saeidi M^{1, 2} ; Yazdani R^{3, 4} ; Babaha F³ ; Falak R^{5, 6} ; Azizi G⁷ ; Taherian M^{5, 6} ; Salami F³ ; Yazdani Y^{1, 2} ; Sadani S⁸ ; Hosseini A⁸ ; Motallebnezhad M^{5, 6} ; Abolhassani H^{3, 9} ; Shekarabi M^{5, 6} Show All Authors

Bagheri Y^{1, 2}
Saeidi M^{1, 2}
Yazdani R^{3, 4}
Babaha F³
Falak R^{5, 6}
Azizi G⁷
Taherian M^{5, 6}
Salami F³
Yazdani Y^{1, 2}
Sadani S⁸
Hosseini A⁸
Motallebnezhad M^{5, 6}
Abolhassani H^{3, 9}
Shekarabi M^{5, 6}
Aghamohammadi A³

Source: European Cytokine Network Published:2021

Abstract

Background: Selective IgA deficiency is the most prevalent form of primary immunodeficiencies. The pathogenesis of the disease is still unknown. Several studies have suggested a defect in B cell responses to IL-10; however, the main reason for this defect has not been reported. Elucidating IL-10 signaling defects and their correlation with clinical manifestations could be helpful for better understanding and treatment of the disease. Methods: In this study, 15 SIgAD patients and 15 age- and sex-matched healthy controls were included. Surface expression of transforming growth factor β receptor II (TGF-β RII), IL-10R and IgA was assessed by flow cytometry in human purified B cells before and after stimulation by IL-10. Protein expression of STAT3, p-STAT3 and SOCS3 was measured by Western blotting analysis. TGF-β and IgA secretion was evaluated by ELISA. Finally, the measurement of B cell apoptosis was performed by flow cytometry. Results: The TGF-βRII expression level was decreased after stimulation with IL-10 in patients compared with controls. Notably, the TGF-β level were higher after stimulation with mCD40L and IL-10 in the control group as compared to stimulation with mCD40L alone. The IgA+ B cell percentage and IgA secretion levels were significantly increased in controls as compared with SIgAD patients. The relative concentration of the total STAT3 was decreased as compared with controls. Conclusion: The defect in IgA production in SIgAD patients could be due to inadequate B cell responses to IL-10 stimulation that probably originate from defective regulation of IL-10-mediated TGF-b ‘symbol’ production TGF-β response by IL-10. Furthermore, it is suggested that the absence of STAT3 protein baseline expression could impair cytokine-mediated signaling such as thatinduced by IL-!0 and IL-21. © 2021, JLE/Springer.

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Maryam Izad (1)

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Style	Citing Format
MLA	Bagheri Y, et al.. "Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency." European Cytokine Network, vol. 33, no. 1, 2021, pp. 1-12.
APA	Bagheri Y, Saeidi M, Yazdani R, Babaha F, Falak R, Azizi G, Taherian M, Salami F, Yazdani Y, Sadani S, Hosseini A, Motallebnezhad M, Abolhassani H, Shekarabi M, Aghamohammadi A (2021). Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency. European Cytokine Network, 33(1), 1-12.
Chicago	Bagheri Y, Saeidi M, Yazdani R, Babaha F, Falak R, Azizi G, Taherian M, et al.. "Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency." European Cytokine Network 33, no. 1 (2021): 1-12.
Harvard	Bagheri Y et al. (2021) 'Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency', European Cytokine Network, 33(1), pp. 1-12.
Vancouver	Bagheri Y, Saeidi M, Yazdani R, Babaha F, Falak R, Azizi G, et al.. Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency. European Cytokine Network. 2021;33(1):1-12.
BibTex	@article{ author = {Bagheri Y and Saeidi M and Yazdani R and Babaha F and Falak R and Azizi G and Taherian M and Salami F and Yazdani Y and Sadani S and Hosseini A and Motallebnezhad M and Abolhassani H and Shekarabi M and Aghamohammadi A}, title = {Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency}, journal = {European Cytokine Network}, volume = {33}, number = {1}, pages = {1-12}, year = {2021} }
RIS	TY - JOUR AU - Bagheri Y AU - Saeidi M AU - Yazdani R AU - Babaha F AU - Falak R AU - Azizi G AU - Taherian M AU - Salami F AU - Yazdani Y AU - Sadani S AU - Hosseini A AU - Motallebnezhad M AU - Abolhassani H AU - Shekarabi M AU - Aghamohammadi A TI - Evaluation of Effective Factors on Il-10 Signaling in B Cells in Patients With Selective Iga Deficiency JO - European Cytokine Network VL - 33 IS - 1 SP - 1 EP - 12 PY - 2021 ER -

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