Up-Regulation of Mir-625-5P Correlates With Suppressed Sox2, Increased Apoptosis, and Cell Cycle Arrest Via the Pi3k/Akt Signalling Pathway in Acute Myeloid Leukaemia Publisher



Ks Kereka Kangup STEVEN ; Sh Mousavi Seyed HADI ; Sh Alizadeh Shaban H ; L Ghaemmaghami LEILA ; G Fakoorizad GHASEM ; Jm Khanmiri Jamal MOTALLEBZADEH
Source: International Journal of Hematology-Oncology and Stem Cell Research Published:2024

Abstract

Background: Up-regulation of the microRNA-625 and abnormal expression of the Sox2 gene have been studied and seen in several tumors. Few reports have also shown the aberrant expression of miR-625 and Sox2 expression in various cancers. Several studies have also confirmed that phosphatidylinositol 3'-kinase /protein kinase B pathways regulate hematological malignancies, including Acute Myeloid Leukemia (AML). Thus, this study aimed to investigate the effects of mir-625 up-regulation on proliferation, apoptosis, and cell cycle by targeting the Sox2 gene via the downstream Akt signaling pathway and cell cycle regulators, such as p21, p27, and cyclin E in the KG-1 cell line. Materials and Methods: Cells obtained from the KG-1 cell line were cultured and transfected with plasmid DNA (miR-625) and scrambled as the control using the Lonza electroporation system. Flow cytometry was used to evaluate cell cycle, proliferation, and apoptosis. Relative gene expression was validated by qRT-PCR. All data were analyzed using graph pad prism 7.01 and REST 2009. Results: KG-1 cells transfected with the mir625-GFP construct showed decreased proliferation, increased apoptosis, and induced cell cycle arrest. Low levels of Sox2, p21, cyclin E, and up-regulation of p27 were confirmed and validated by qRT-PCR (P < 0.05). Conclusion: MiR-625 can be a promising approach to aid in the treatment of AML. However, further studies are required in this field. © 2024 Elsevier B.V., All rights reserved.