Comparative Effects of Aluminium on Serum, Liver and Brain High and Low Molecular Weight Alkaline Phosphatase in Rats Publisher



Moshtaghie AA¹ ; Ani M¹ ; Mirhashemi SM¹ Show Affiliations
Source: Journal of Medical Sciences Published:2006

Abstract

The relationship between aluminium treatment and changes in the concentration of serum, liver and brain high- and low molecular weight alkaline phosphatase has been investigated in this manuscript. Results obtained showed that every other day intrapritoneally injection of 186 μmol kg-1 of aluminium (A1C13.6H2O), in male rats for 2 weeks resulted in decreasing the level of liver and brain alkaline phosphatase by 14.9 and 9.9%, respectively, whereas an elevation of serum levels of this enzyme by 21.1% was seen in comparison to untreated controls (p<0.05). Long-term exposure to 74.5 μmol kg-1 of this salt, showed a statistically significant reduction in liver and brain levels of alkaline phosphatase by 15.8 and 12.3%, respectively and an increment in serum activity of the enzyme by 30.9% in compared to control group (p<0.05). Using gel filtration chromatography technique with sepbacryl S300 showed that, in comparison to control groups, serum and liver homogenate from aluminium treated groups had a significant level of high molecular weight alkaline phosphatase, which might be considered as a potential biomarker for aluminium toxicity.