Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Incorporation of Chitosan Nanoparticles Into Silk Fibroin-Based Porous Scaffolds: Chondrogenic Differentiation of Stem Cells Publisher

Summary: Scientists report a new scaffold boosts stem cell growth for cartilage repair. It’s stronger and supports key cartilage genes, promising better joint healing. #TissueEngineering #CartilageRepair

Naeimi M1, 2 ; Rafienia M3 ; Fathi M2, 4 ; Janmaleki M5 ; Bonakdar S6 ; Ebrahimianhosseinabadi M7
Authors

Source: International Journal of Polymeric Materials and Polymeric Biomaterials Published:2016


Abstract

A silk fibroin-chondroitin sulfate-sodium alginate (SF-CHS-SA) porous scaffold containing chitosan nanoparticles (NPs) was investigated. The proliferation of adipose-derived stem cells (ASCs) was studied by SEM, fluorescent microscopy, alcian blue staining, dimethylmethylene blue assay, and real-time polymerization chain reaction. The results showed that incorporation of NPs into the scaffold improved compressive modulus (5.6 ± 0.15 MPa). The amount of glycosaminoglycan expression of the ASCs was reached to 8.9 ± 0.3 g/mL. The gene expressions of aggrecan, collagen II, and SOX9 of the ASCs were significantly improved. This study revealed that the prepared scaffold can be used as a substrate for cartilage tissue engineering. © 2016 Taylor & Francis Group, LLC.
Related Docs
View other Related Docs
1. Comparison Between Chondrogenic Markers of Differentiated Chondrocytes From Adipose Derived Stem Cells and Articular Chondrocytes in Vitro, Iranian Journal of Basic Medical Sciences (2013)
2. Evaluation Avocado Soybean Unsaponifiables Loaded in Poly (Lactic-Co-Glycolic) Acid/Avocado Soybean Unsaponifiables-Fibrin Nanoparticles Scaffold (New Delivery System) Is an Effective Factor for Tissue Engineering, Advanced Biomedical Research (2021)
3. Evaluation of the Effects of Glucosamine Sulfate on Poly(3- Hydroxybutyrate) -Chitosan/Carbon Nanotubes Electrospun Scaffold for Cartilage Tissue Engineering Applications, Polymer-Plastics Technology and Materials (2022)
Experts (# of related papers)
View all Related Experts
Batool Hashemibeni (11)
Saeed Karbasi (8)
Incorporation of Chitosan Nanoparticles Into Silk Fibroin-Based Porous Scaffolds: Chondrogenic Differentiation of Stem Cells
Other Related Docs
4. Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold, International Journal of Preventive Medicine (2014)
5. Impact of Fibrin on the Chondrogenic Avocado Soybean Unsaponifiables on Poly (Lactic-Co-Glycolic) Acid Scaffold, Biointerface Research in Applied Chemistry (2021)
6. Evaluation of the Effects of Chitosan Nanoparticles on Polyhydroxy Butyrate Electrospun Scaffolds for Cartilage Tissue Engineering Applications, International Journal of Biological Macromolecules (2023)
7. Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine, Journal of Biomaterials Science, Polymer Edition (2024)
8. Evaluation of the Effects of Decellularized Wharton Jelly Nanoparticles on Polyhydroxy Butyrate-Chitosan Electrospun Scaffolds for Cartilage Tissue Engineering Applications, Journal of Polymers and the Environment (2025)
9. A Comparison Between Cell Viability of Chondrocytes on a Biodegradable Polyester Urethane Scaffold and Alginate Beads in Different Oxygen Tension and Ph, Iranian Polymer Journal (English Edition) (2005)
10. An Animal Model Study of Osteochondral Defect Repair by Human Adipose Stem Cells and Pomegranate Fruit Hydroalchoholic Extract, Avicenna Journal of Phytomedicine (2023)
11. Cartilage Tissue Formation From Human Adipose-Derived Stem Cells Via Herbal Component (Avocado/Soybean Unsaponifiables) in Scaffold-Free Culture System, Dental Research Journal (2020)
12. Regeneration of Rat Sciatic Nerve Using Plga Conduit Containing Rat Adscs With Controlled Release of Bdnf and Gold Nanoparticles, Journal of Molecular Neuroscience (2021)
13. Fabrication and Characterization of Chitosan-Gelatin/Single-Walled Carbon Nanotubes Electrospun Composite Scaffolds for Cartilage Tissue Engineering Applications, Polymers for Advanced Technologies (2022)
14. Investigation and Comparison of the Effect of Tgf-Β3, Kartogenin and Avocado/Soybean Unsaponifiables on the In-Vitro and In-Vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold, Iranian Journal of Pharmaceutical Research (2021)
15. The Effect of Pomegranate Extract on Producing Type Ii Collagen in Differentiation of Adipose-Derived Stem Cells Into Chondrocytes, Journal of Isfahan Medical School (2018)
16. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
17. Evaluation of Mechanical Properties and Cell Viability of Poly (3-Hydroxybutyrate)-Chitosan/Al2o3nanocomposite Scaffold for Cartilage Tissue Engineering, Journal of Medical Signals and Sensors (2019)
18. Biological Evaluation of the Effects of Hyaluronic Acid on Poly (3-Hydroxybutyrate) Based Electrospun Nanocomposite Scaffolds for Cartilage Tissue Engineering Application, Materials Technology (2020)
19. Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering, Tissue Engineering - Part B: Reviews (2019)
20. Comparing the Effects of Transforming Growth Factor Beta1 (Tgf-ß1) and Piascledine on the Expression of Collagen Ii, X and Aggrecan Genes in Chondrogenesis of Human Adipose-Derived Stem Cells in Fibrin Alginate Composite Scaffold, Journal of Isfahan Medical School (2016)