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A Comparison Between Cell Viability of Chondrocytes on a Biodegradable Polyester Urethane Scaffold and Alginate Beads in Different Oxygen Tension and Ph



Karbasi S1, 2 ; Mirzadeh H1 ; Orang F1 ; Urban JPG3
Authors
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Authors Affiliations
  1. 1. Faculty of Biomedical Engineering, Amir Kabir University of Technology, Tehran, P.O. Box: 15875/4413, Iran
  2. 2. Biomedical Engineering Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, P.O. Box: 81745/313, Iran
  3. 3. Disc Group, University Laboratory of Physiology, Oxford University, Oxford, P.O.Box: OX13PT, United Kingdom

Source: Iranian Polymer Journal (English Edition) Published:2005

Abstract

Cartilage is a tissue that has a low potential for self-repair. One of the methods for improvement of regeneration and metabolism in cartilge, is to stimulate physical factors on chondrocytes as cartilage based cells. In this research, two physical factors, oxygen tension and pH, were changed to measure the cell viability of chondrocytes on Degrapol®, as a biodegradable polyurethane (DBS), and alginate scaffolds and cell viability onto these substrates are compared. The results showed that, physical factors like oxygen and pH could change cell viability. After 3 days cell culture for both types of scaffolds, the cell viability was higher with 5% O2 and pH=7.4 and it was not dependent on the type of scaffolds. Our results showed that cell viability on alginate beads is more than DBS scaffold for different conditions.
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