Development of a Latex Agglutination Test As a Simple and Rapid Method for Diagnosis of Trichomonas Vaginalis Infection



Darani HY¹ ; Ahmadi F² ; Zabardast N² ; Yousefi HA³ ; Shirzad H⁴ Show Affiliations
Source: Avicenna Journal of Medical Biotechnology Published:2010

Abstract

Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex agglutination tests. Sensitivity and specificity of latex test was determined considering culture as golden standard. Sensitivity and specificity of latex agglutination test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex agglutination test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex agglutination test developed in this study, further work is recommended to improve the test. Copyright © 2010, Avicenna Journal of Medical Biotechnology. All rights reserved.