Effects of Vitamin D Supplementation on 8-Hydroxydeoxy Guanosine and 3-Nitrotyrosine in Patients With Type 2 Diabetes: A Randomized Clinical Trial Publisher



Damghanian P¹ ; Javanbakht MH¹ ; Honarvar NM¹ ; Yousefirad E² ; Mohammadi H³ ; Zarei M¹ ; Djalali M¹
Source: Progress in Nutrition Published:2019

Abstract

Objective: This study was done to assess the effects of Vitamin D supplementation on oxidative stress biomarker in p patients with Type 2 Diabetes Mellitus (T2DM). Methods: This randomized doubleblind clinical trial was done with 35 type 2 diabetic participants. The Vitamin D group (n= 17) and the placebo group (n= 18) were stratified for BMI and age at the baseline before random allocation. The Vitamin D group received 4000 IU (100μg) Vitamin D3/day whereas the placebo group received matching placebo. The effect of Vitamin D3 on 8-OHdG and 3-nitrotyrosine was measured by ELIZA kit at the beginning and at the end of the study. Results: After two months supplementation, the mean concentration of serum 8-OHDG had significantly decreased (P=0.015) in Vitamin D group. The mean concentration of 3-NT decreased in both groups, whereas this reduction was significant only in Vitamin D group (p=0.027). Conclusions: Vitamin D supplementation at the dose of 100μg for 2 months had beneficial effects on oxidative stress T2DM management. Results from this trial showed that there were beneficial effects associated with the use of Vitamin D supplementation, specifically reducing 8-OHDG and 3-NT concentrations. © Mattioli 1885.