Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! By
3D Printed Phb-Dextran-Whitlockite Porous Construct Coated With Sildenafil-Loaded Nanofibers: A Hybrid Scaffold for Craniofacial Reconstruction Publisher Pubmed



La Nazar Lara ALI ; Ss Alsalman Sarah SAMEER ; Sh Torki Sumyah HASAN ; Mh Almusawi Mastafa HJ ; A Najafinezhad ALIAKBAR ; P Noory PARASTOO ; Es Rajab Eslah SHAKIR ; N Khosravi NEGIN ; S Talebi SINA ; F Azamian FARIBA
Authors

Source: International Journal of Biological Macromolecules Published:2025


Abstract

In this study, a novel hybrid scaffold comprising 3D-printed porous polyhydroxybutyrate (PHB), dextran (Dex), and magnesium-doped whitlockite (WL) nanoparticles was developed, which were further enhanced with an electrospun nanofibrous coating composed of Dex and Pluronic F127 (F127) loaded with Sildenafil (Sil) for use in craniofacial regeneration. This design was intended to improve the solubility of sildenafil and enable controlled release. Scanning electron microscopy (SEM) revealed a well-integrated structure between the 3D-printed strands and electrospun nanofibers. The scaffold exhibited sustained release of Sil over 28 days, with mechanical testing showing a compressive strength of 3.70 ± 0.33 MPa and an elastic modulus of 49.04 ± 4.62 MPa. Non-toxicity was confirmed via MTT assay on the MG63 cell line, and qRT-PCR results indicated significantly higher expression levels of collagen I, RUNX2, osteocalcin, VEGF, and CD31 markers associated with osteogenesis and angiogenesis. Following implantation in a rat calvarial defect model, the scaffold demonstrated robust osteogenic activity and new bone tissue formation over an eight-week period. This innovative scaffold design offers a promising solution for overcoming the challenges in craniofacial defect repair by integrating bioactive materials with advanced drug delivery systems, leading to more effective tissue regeneration strategies. © 2025 Elsevier B.V., All rights reserved.
Related Docs
View other Related Docs
1. Optimization and Characterization of Polyhydroxybutyrate/Lignin Electro-Spun Scaffolds for Tissue Engineering Applications, International Journal of Biological Macromolecules (2022)
2. Incorporation of Multi-Walled Carbon Nanotubes Into Electrospun Pcl/Gelatin Scaffold: The Influence on the Physical, Chemical and Thermal Properties and Cell Response for Tissue Engineering, Materials Technology (2020)
3. Electrospun Polycaprolactone/Lignin-Based Nanocomposite As a Novel Tissue Scaffold for Biomedical Applications, Journal of Medical Signals and Sensors (2017)
Experts (# of related papers)
View all Related Experts
Saeed Karbasi (10)
Mohammad Rafienia (4)
Other Related Docs
4. Fabrication and Evaluation of Pcl/Collagen I-Based 3D Nanofibrous Scaffolds by Mold-Assisted Electrospinning for Tissue Engineering and Regenerative Medicine, Journal of Applied Polymer Science (2025)
5. Evaluation of the Effects of Keratin on Physical, Mechanical and Biological Properties of Poly (3-Hydroxybutyrate) Electrospun Scaffold: Potential Application in Bone Tissue Engineering, European Polymer Journal (2020)
6. Novel Electrospun Polyurethane Scaffolds Containing Bioactive Glass Nanoparticles, Bioinspired, Biomimetic and Nanobiomaterials (2020)
7. Hybrid Nano-Micro Scaffolds for Cartilage Tissue Engineering: Integrating Pcl-Dwjm-Mwcnts on Chemically Modified Silk Fibroin, Journal of Polymers and the Environment (2025)
8. Physical, Mechanical and Biological Evaluation of Poly (3-Hydroxybutyrate)-Chitosan/Mwnts As a Novel Electrospun Scaffold for Cartilage Tissue Engineering Applications, Polymer-Plastics Technology and Materials (2020)
9. Ultra-Thin Electrospun Nanocomposite Scaffold of Poly (3-Hydroxybutyrate)-Chitosan/Magnetic Mesoporous Bioactive Glasses for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2024)
10. The Electrospun Poly(Ε-Caprolactone)/Fluoridated Hydroxyapatite Nanocomposite for Bone Tissue Engineering, Polymers for Advanced Technologies (2020)
11. Sustained Release Investigation of Curcumin and Ciprofloxacin on Coaxial Electrospun Nanocomposite Scaffold of Poly (3-Hydroxybutyrate)-Poly Glycerol Sebacate: An Approach for Skin Regeneration, Emergent Materials (2025)
12. Synthetic-Based Blended Electrospun Scaffolds in Tissue Engineering Applications, Journal of Materials Science (2022)
13. Artificial Neural Network for Modeling the Elastic Modulus of Electrospun Polycaprolactone/Gelatin Scaffolds, Acta Biomaterialia (2014)
14. Fabrication and Characterization of Novel Polyhydroxybutyrate-Keratin/Nanohydroxyapatite Electrospun Fibers for Bone Tissue Engineering Applications, International Journal of Biological Macromolecules (2022)
15. An Antibacterial Multi-Layered Scaffold Fabricated by 3D Printing and Electrospinning Methodologies for Skin Tissue Regeneration, International Journal of Pharmaceutics (2023)
16. Graphene and Hydroxyapatite-Enhanced Gelatin/Pgs Electrospun Nanocomposite Scaffolds for Neural Tissue Engineering, Journal of Polymers and the Environment (2025)
17. Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-Hydroxyapatite and Vitamin D3, Journal of Isfahan Medical School (2017)
18. Scaffold Percolative Efficiency: In Vitro Evaluation of the Structural Criterion for Electrospun Mats, Journal of Materials Science: Materials in Medicine (2010)
19. Hybrid Nano-Micro Scaffolds for Cartilage Tissue Engineering: Integrating Pcl-Dwjm-Mwcnts on Chemically Modified Silk Fibroin, Journal of Polymers and the Environment (2025)
20. Biomimetic Vegf-Loaded Bilayer Scaffold Fabricated by 3D Printing and Electrospinning Techniques for Skin Regeneration, Materials and Design (2024)