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Cytotoxicity Evaluation of Synthesized Silver Nanoparticles by a Green Method Against Ovarian Cancer Cell Lines Publisher



Abbasi S1 ; Ilhan A2 ; Jabbari H3 ; Javidzade P4 ; Safari M5 ; Zadeh FA6
Authors
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Authors Affiliations
  1. 1. Department of medical Education, Kosar hospital, Lorestan, Borujerd, Iran
  2. 2. Department of Medical Biochemistry, Faculty of Medicine, Cukurova University, Adana, Turkey
  3. 3. Department of Chemistry, Payame Noor University, P.O. Box 19395-, Tehran, 4697, Iran
  4. 4. Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  5. 5. Department of Urology, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Surgery, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Nanomedicine Research Journal Published:2022


Abstract

Objective(s): In recent years, the attention of many was focused on the features of the Green Synthesis process, such as its simplicity, cost-effectiveness, and environmental friendliness. This study investigates the feasibility of manufacturing silver nanoparticles from Malva sylvestris L aqueous extract and evaluates its anticancer effect on ovarian cancer cell lines OVCAR-3 and SK-OV-3. Methods: The aqueous extract of Malva sylvestris L proved to be capable of synthesizing silver nanoparticles (Ag-NPs) and the success of the procedure was affirmed through the results of characterizing analyzes such as UV-Vis spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Additionally, we assessed the anti-cancer impacts of synthesized Ag-NPs on OVCAR-3 and SK-OV-3 cell lines by the means of MTT assay. The Bax, Bcl-2, caspase 3, and caspase 8 expression at mRNA levels were also estimated using real-time PCR. Results: The appearance of an absorption peak at 462 nm using a spectrophotometer signified the complete synthesis of silver nanoparticles. The electron microscope images showed that the nanoparticles were spherical and had an average size of about 74.4 nm. Ag-NPs at the concentration (10-200nM) inhibited the proliferation of OVCAR-3 and SK-OV-3 cell lines. Moreover, treatment resulted in down-regulating Bcl-2 while up-regulating Bax and caspase 3 and caspase 8 expression. Conclusions: In this study, synthesized Ag-NPs using Malva sylvestris L could induce a robust anti-cancer effect on ovarian cells in vitro by improving Bax/Bcl-2 ratio and triggering the apoptosis pathway. © 2022 International Journal of Health Sciences. All rights reserved.
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