Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction



Bahrambeigi V¹ ; Rafiee L² ; Javanmard SH³ ; Salehi R⁴ ; Tajedini MH⁵ ; Daraei A⁶
Source: Journal of Isfahan Medical School Published:2012

Abstract

Background: Micro ribonucleic acids (miRNA) are a type of ribonucleic acids with 20-24 nucleotides. They seem to have an important regulating role in cells. MiR-211 is an miRNA whose expression decreases in cancer cells, especially human melanoma cells. It is of high importance in suppression of tumor growth and invasion. Up to now, no study has evaluated miR-211 expression in murine melanoma cells for in vivo and in vitro conditions. We evaluated and compared the expression of miR-211 in murine melanoma cell line and melanoma tumors using real-time polymerase chain reaction (PCR). Methods: Melanoma B16F10 cell line was cultured. Subcutaneous melanoma tumors were induced by injecting this line to C57 mice. Melanoma cell line and melanoma tumors were lysed and miRNAs were extracted. After complementary deoxyribonucleic acid (cDNA) synthesis, real-time PCR of samples was performed using miR-211 primers. Data was statistically analyzed by Student's t-test. Findings: Average expression of miR-211 in melanoma cell line was significantly higher than melanoma tumors. Conclusion: Due to the lower expression of miR-211 in murine melanoma tumors in comparison to murine melanoma cells, miRNA may be involved in invasive properties of melanoma cells in the mouse model of melanoma.