Effect of Intra-Hippocampal Injection of Human Recombinant Growth Hormone on Synaptic Plasticity in the Nucleus Basalis Magnocellularis-Lesioned Aged Rats; [Efeito Da Injecao Intrahipocampal De Hormonio Do Crescimento Humano Recombinante Sobre a Plasticidade Sinaptica Em Ratos Envelhecidos Lesados Do Nucleo Basalis Magnocellularis] Publisher Pubmed



Malek M¹ ; Sarkaki A^{2, 4} ; Zahediasl S³ ; Rajaei Z¹ ; Farbood Y^{2, 4}
Source: Arquivos de Neuro-Psiquiatria Published:2017

Abstract

In this study, we proposed that administration of hippocampal growth hormone in ageing animals with growth hormone deficiency can compensate long-term potentiation and synaptic plasticity in nucleus basalis magnocellularis (NBM)-lesioned rats. Aged male Wistar rats were randomly divided into six groups (seven in each) of sham-operated healthy rats (Cont); NBM-lesioned rats (L); NBM-lesioned rats and intrahippocampal injection of growth hormone vehicle (L + Veh); NBM-lesioned and intrahippocampal injection of growth hormone (10, 20 and 40 μg.2 μl-1) (L + GH). In vivo electrophysiological recording techniques were used to characterize maintenance of long-term potentiation at distinct times (1, 2, 3, 24 and 48 hours) after high-frequency stimulation. The population spike was enhanced significantly for about 48 hours following tetanic stimulation in rats treated with a dose-dependent growth hormone compared to the vehicle group (p < 0.05), possibly through neuronal plasticity and neurogenesis in affected areas. © 2017, Associacao Arquivos de Neuro-Psiquiatria. All rights reserved.