Oxidized Ldl Metabolites With High Family Risk for Premature Cardiovascular Disease Publisher Pubmed



Kelishadi R¹ ; Nadery GA¹ ; Asgary S¹ Show Affiliations
Source: Indian Journal of Pediatrics Published:2002

Abstract

Objective : Considering the importance of primary prevention of Cardiovascular Disease (CVD) from childhood, especially in children with high family risk for premature atherosclerosis, and also the importance of oxidized LDL in the process of atherosclerosis, the main metabolites of ox-LDL i.e. Malondialdehyde (MDA) and Conjugated diene (CDE) have been measured in children of high risk families and compared with a control group. Methods : Children and adolescents (6-18 years) of parents with premature myocardial infarction (MI ≤ 55y in men and ≤ 65y in women), were selected as the case group. The control group included neighbors of the case group matched for age and socioeconomic status. All samples have been selected by simple random sampling. Both the case and control groups were divided in two subgroups : those with a total cholesterol and/or LDL-C ≥95th centile and those with normal lipid levels. Each subgroup consisted of 32 subjects, so 128 subjects were studied (64 in the case and 64 in the control group). MDA and CDE were measured by spectrophotometry using molar absorbivity. Data were analyzed by SPSSv10/Win software using ANOVA, Bon-ferroni, Scheffe-Duncan, Tukey-HSD, and the Student's t-test. Result : The mean MDA value in the case and control groups was significantly different (1.84 ± 0.43 vs. 1.67 ± 0.41 μmol/L, p=0.03), but this difference was not significant regarding the mean CDE level (0.50 ± 0.05 vs. 0.47 ± 0.04 μmol/ L, p>0.05). The mean MDA level in the case group with hyperlipidemia was significantly higher than that in the case group without hyperlipidemia (1.985 ± 0.516 vs. 1.690 ± 0.366, μmol/L, P=0.02) and also higher than control group with or without hyperlipidemia (1.985 ± 0.516 vs. 1.720 ± 0.389, 1.615 ± 0.429 μmol/L respectively, P<0.05). The mean CDE level in the case group with hyperlipidemia was significantly higher than the case group without hyperlipidemia (0.542 ± 0.034 vs. 0.494 ± 0.049 μmol/L, P=0.04) and higher than the control group with or without hyperlipidemia (0.542 ± 0.034 vs. 0.464 ± 0.051, 0.484 ± 0.048 μmol/L respectively, p<0.05). In case boys with hyperlipidemia, the mean MDA (2.03 ± 0.2 μmol/L) and the mean of CDE (0.56 ± 0.04 μmol/L) was significantly higher than other subgroups (P<0.05). Conclusion : Considering the increased susceptibility of LDL to oxidation in children with high family risk for premature CVD, special attention should be paid to consumption of foods and seasoning containing antioxidants from childhood especially in high risk families.