Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Silymarin on the Proliferating of the Activated Human T Cells in Vitro



Almasi E1 ; Eskandari N1 ; Gharagozloo M1 ; Almasi A2 ; Ostadi V3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Clinical Research Development Unit, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
  3. 3. Flow Cytometry Lab and Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: The widespread use of herbal medicines, because of their protective effects on various organs, has been shown in many studies. Silymarin is a flavonolignan complex isolated from the milk thistle (Silybum marianum L. Gaertn), has been clinically used for its anti-inflammatory and hepatoprotective effects. Recently, immunomodulating effects of this plant have considered by the researchers. This study aimed to investigate the effect of silymarin and dimethyl sulfoxide (DMSO) on the human T lymphocyte proliferation under the cell culture conditions. Methods: Peripheral blood mononuclear cells (PBMCs) from the healthy individuals were activated with Con A (10 μg/ml) and treated with silymarin at different concentrations (0.001, 0.01, 0.1, 1, 10, 100 and 200 μM). Then, cells were incubated for five days at 37° C with 5% CO2 for proliferation assay using the carboxyfluorescein succinimidyl ester (CFSE) dye and for viability analysis using flow cytometry. We analyzed the data using the Graph pad prism (version 6) software. Findings: Silymarin had the ability to inhibit the T cell proliferation in vitro. Moreover, 100 μM and 200 μM of silymarin had more inhibitory effect on T cells (P < 0.001). Conclusion: We conclude that silymarin exerts immunosuppression effects, and it could be used in therapeutic situations with fewer side effects compared with the other immunosuppressive drugs. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Inhibition of Apoptosis and Proliferation in T Cells by Immunosuppressive Silymarine, Iranian Journal of Allergy, Asthma and Immunology (2017)
2. Silymarin Impacts on Immune System As an Immunomodulator: One Key for Many Locks, International Immunopharmacology (2017)
3. Silymarin Inhibits Cell Cycle Progression and Mtor Activity in Activated Human T Cells: Therapeutic Implications for Autoimmune Diseases, Basic and Clinical Pharmacology and Toxicology (2013)
4. Assessment of Pi3k/Akt Signaling Pathway in Activated Human T Lymphocytes in Vitro, Journal of Isfahan Medical School (2012)
5. Silymarin Suppress Cd4+ T Cell Activation and Proliferation: Effects on Nf-Κb Activity and Il-2 Production, Pharmacological Research (2010)
6. Immunosuppressive Effect of Silymarin on Mitogen-Activated Protein Kinase Signalling Pathway: The Impact on T Cell Proliferation and Cytokine Production, Basic and Clinical Pharmacology and Toxicology (2013)
7. Immunomodulatory Effects of Silymarin in Patients With Β-Thalassemia Major, International Immunopharmacology (2013)
8. The Effect of Silymarin on the Expression of Chemokine Receptors in T Helper 1 (Th1) Cells, Journal of Isfahan Medical School (2014)
Experts (# of related papers)
View all Related Experts
Nafiseh Esmaeil (8)
Nahid Eskandari (5)
Other Related Docs
9. Immunoregulatory Effects of Silymarin on Proliferation and Activation of Th1 Cells Isolated From Newly Diagnosed and Ifn-ß 1B -Treated Ms Patients, Inflammation (2019)
10. Comparing In-Vitro Effects of Two Immunosuppressive Drugs on the Expression of Foxp3 From Naive Cd4+ T Cells, Journal of Isfahan Medical School (2015)
11. The Favorable Impacts of Silibinin Polyphenols As Adjunctive Therapy in Reducing the Complications of Covid-19: A Review of Research Evidence and Underlying Mechanisms, Biomedicine and Pharmacotherapy (2022)
12. The Effects of Silymarin and Cyclosporine a on the Proliferation and Cytokine Production of Regulatory T Cells, Immunological Investigations (2019)
13. Expression of Nkg2d Receptors in Cd3+And Cd56+Cells in Blood Samples of Pre-Eclampsia Patients and the Effect of Silymarin on Their Activity in Vitro, Journal of Isfahan Medical School (2022)
14. Evaluating the Safety and Efficacy of Silymarin in Β-Thalassemia Patients: A Review, Hemoglobin (2015)
15. The Influence of Fructose, Glucose and Sucrose on Flavonolignans Formation in Silybum Marianum Callus Culture, Journal of Medicinal Plants (2008)
16. The Effects of Silymarin Consumption on Inflammation and Oxidative Stress in Adults: A Systematic Review and Meta-Analysis, Inflammopharmacology (2024)
17. Thymoquinone Mitigates Diclofenac-Induced Hepatorenal Toxicity in Male Wistar Rats by Balancing the Redox State and Modulating Bax/Bcl-2/Caspase-3 Apoptotic Pathways and Nf-Κb Signaling, Research in Pharmaceutical Sciences (2025)
18. Combined Therapy of Silymarin and Desferrioxamine in Patients With Β-Thalassemia Major: A Randomized Double-Blind Clinical Trial, Fundamental and Clinical Pharmacology (2009)
19. The Influence of Iron Loading and Iron Chelation on the Proliferation and Telomerase Activity of Human Peripheral Blood Mononuclear Cells, Iranian Journal of Immunology (2009)
20. Trans-Differentiation of Mouse Mesenchymal Stem Cells Into Pancreatic Β-Like Cells by a Traditional Anti-Diabetic Medicinal Herb Medicago Sativa L, Journal of Traditional and Complementary Medicine (2022)
21. Silybum Marianum: Beyond Hepatoprotection, Journal of Evidence-Based Complementary and Alternative Medicine (2015)
22. The Effects of Herbal Medicines on Cancer Therapy-Induced Oral Mucositis: A Literature Review, Phytotherapy Research (2022)
23. Preparation and Characterisation of a New Form of Silymarin As a Potential Antidiabetic Agent in the Adult Male Rat, Archives of Physiology and Biochemistry (2023)
24. The Significant Impact of Apigenin on Different Aspects of Autoimmune Disease, Inflammopharmacology (2018)
25. A Randomized Double-Blind, Placebo-Controlled Study of Therapeutic Effects of Silymarin in Β-Thalassemia Major Patients Receiving Desferrioxamine, European Journal of Haematology (2013)
26. The Effect of Medicinal Plants on Cirrhosis: A Systematic Review of Clinical Trials, Phytotherapy Research (2023)
27. Effects of Silymarin on the Proliferation and Glutathione Levels of Peripheral Blood Mononuclear Cells From Β-Thalassemia Major Patients, International Immunopharmacology (2006)
28. Methoxylated Flavones From Salvia Mirzayanii Rech. F. and Esfand With Immunosuppressive Properties, Iranian Journal of Pharmaceutical Research (2015)
29. The Cytotoxic and Pro-Apoptotic Effects of Various Extracts of Allium Giganteum on Mcf-7 and Hela Cell Lines, Journal of Isfahan Medical School (2021)
30. Effects of Silymarin Supplementation on Blood Lipids: A Systematic Review and Meta-Analysis of Clinical Trials, Phytotherapy Research (2019)
31. Significant Immunomodulatory and Hepatoprotective Impacts of Silymarin in Ms Patients: A Double-Blind Placebo-Controlled Clinical Trial, International Immunopharmacology (2021)
32. Hepatotoxicity Effects of Medicinal Plants, New Armenian Medical Journal (2024)
33. The Effects of Allium Hirtifolium Boiss. on Cell-Mediated Immune Response in Mice, Iranian Journal of Pharmaceutical Research (2003)
34. Evaluation of in Vitro Cytotoxic Effects of Juniperus Foetidissima and Juniperus Sabina Extracts Against a Panel of Cancer Cells, Iranian Journal of Pharmaceutical Research (2009)
35. Effects of Silybum Marianum on Patients With Chronic Hepatitis C, Journal of Research in Medical Sciences (2011)
36. Inhibitory Potential of Pure Isoflavonoids, Red Clover, and Alfalfa Extracts on Hemoglobin Glycosylation, ARYA Atherosclerosis (2015)
37. Silymarin Restores Regulatory T Cells (Tregs) Function in Multiple Sclerosis (Ms) Patients in Vitro, Inflammation (2019)
38. The Effects of Chloroform, Ethyl Acetate and Methanolic Extracts of Brassica Rapa L. on Cell-Mediated Immune Response in Mice, Research in Pharmaceutical Sciences (2013)
39. Hepatoprotective Effect of Chichorium Intybus L. on Liver Toxicity in Rat, Journal of Medicinal Plants (2006)
40. The Hepatorenal Protective Effects of Silymarin in Cancer Patients Receiving Chemotherapy: A Randomized, Placebo-Controlled Trial, BMC Complementary Medicine and Therapies (2024)
41. Effects of Lactulose and Silymarin on Liver Enzymes in Cirrhotic Rats, Canadian Journal of Physiology and Pharmacology (2017)
42. Preventive Effect of Novel Nanomissile of Silymarin on Liver Injury Induced by Carbon Tetrachloride in Rat, Scientific Journal of Kurdistan University of Medical Sciences (2021)
43. The Effects of Garlic Extract on Il-17A Il-17F and Ifnγ Mrna Expression in Peripheral Blood Mononuclear Cells of Multiple Sclerosis Patients, Journal of Isfahan Medical School (2022)
44. Serum Levels of Tgfβ, Il-10, Il-17, and Il-23 Cytokines in Β-Thalassemia Major Patients: The Impact of Silymarin Therapy, Immunopharmacology and Immunotoxicology (2014)
45. Silymarin for the Prevention of Contrast-Induced Nephropathy: A Placebo-Controlled Clinical Trial, International Journal of Preventive Medicine (2016)