Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Inhibitory Effects of Lactococcus Lactis and Its Supernatant on Listeria Monocytogenes Publisher Pubmed



Zamani S1 ; Narimisa N2 ; Kalani BS4, 5 ; Moghadampour M3 ; Jamkarani SK6 ; Irajian G2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Faculty of Agricultural Sciences and Food Industries, Institute of Higher Education Khazar Mahmudabad, Mazandaran, Iran
  2. 2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
  5. 5. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
  6. 6. Department of Microbiology, Ghom Branch, Islamic Azad University, Ghom, Iran

Source: Clinical Laboratory Published:2021


Abstract

Background: The application of biological compounds generated by lactic acid bacteria, especially Lactococcus lactis, is recently considered to be a natural preservative for improving quality and health of food. The purpose of this study is to investigate the inhibitory potential of L. lactis supernatant on the expression of inlA, plc, and hly genes related to L. monocytogenes virulence capacity. Methods: L. lactis was cultured under anaerobic conditions for 16 - 18 hours. The supernatant and live bacteria were then separated by centrifuge. The anti-listeria effects of L. lactis and supernatant were measured using the agar diffusion technique, and the effect on the expression of the virulence-related genes was calculated by real-time PCR. Also, the effects of live bacteria and its supernatant on the microbial count of milk and sausage infected by L. monocytogenes was evaluated by the colony count assay. Results: After 24 hours, the highest non-growing hole diameter was obtained in the presence of acidic supernatant (pH = 3.5). The microbial count showed the inhibitory effect on the eighth day after incubation with L. lactis. qPCR data revealed a down-regulation of virulence-related genes of inlA (8 fold), hly (6 fold), and plc (1 fold) in L. monocytogenes after 24-hour incubation with the supernatant. Conclusions: Our findings showed that the supernatant of L. lactis has an effective inhibitory role in the growth of L. monocytogenes. In the presence of supernatant, among plc, inlA and hly genes, the expression of inlA and hly genes decreased after 2 hours, which could indicate the molecular inhibitory mechanism of L. lactis in L. monocytogenes. © 2021 Verlag Klinisches Labor GmbH. All rights reserved.
Related Docs
View other Related Docs
1. Prevalence of Listeria Species in Raw Milk and Traditional Dairy Products in Isfahan, Iran, International Journal of Environmental Health Engineering (2014)
2. Prevalence of Listeria Species in Raw Milk in Isfahan, Iran, Journal of Isfahan Medical School (2012)
3. Enterotoxigenicity, Distribution of Enterotoxigenic Genes and Antibiotic Resistance Pattern of Staphylococcus Aureus Isolated From Traditional Sweet, Tropical Biomedicine (2018)
4. Investigating the Antagonistic Effect of Indigenous Probiotics on Carbapenem-Resistant Pseudomonas Aeruginosa Strains, BioMed Research International (2023)
5. Frequency and Antibiotic Resistance Pattern of Salmonella Spp. Isolated From Traditional Dairies and Raw Milks Collected in Yazd Province, Iran, Iranian Journal of Microbiology (2024)
6. Inhibitory Effects of Ethanolic, Methanolic, and Hydroalcoholic Extracts of Olive (Olea Europaea) Leaf on Growth, Acid Production, and Adhesion of Streptococcus Mutans, Dental Research Journal (2020)
7. Probiotic Potential, Antiproliferative, and Proapoptotic Activities of Lactobacillus Fermentum Isolated From Breast Milk, Advanced Biomedical Research (2023)
8. Microbial Safety and Chemical Characteristics of Sausage Coated by Chitosan and Postbiotics Obtained From Lactobacillus Bulgaricus During Cold Storage, Scientific Reports (2025)
Experts (# of related papers)
View all Related Experts
Hossein Fazeli (4)
Rasoul Salehi (4)
Other Related Docs
9. Lactobacillus Acidophilus-Derived Biosurfactant Effect on Gtfb and Gtfc Expression Level in Streptococcus Mutans Biofilm Cells, Brazilian Journal of Microbiology (2011)
10. Chemical Composition and Antibacterial Activities of Sumac Fruit (Rhus Coriaria) Essential Oil on Dental Caries Pathogens, Open Microbiology Journal (2020)
11. Bacillus Cereus in Infant Foods: Prevalence Study and Distribution of Enterotoxigenic Virulence Factors in Isfahan Province, Iran, The Scientific World Journal (2013)
12. Biofilm-Producing Ability of Staphylococcus Spp Isolated From Different Foodstuff Products, Annali di Igiene Medicina Preventiva e di Comunita (2019)
13. Stability Evaluation of Freeze-Dried Lactobacillus Paracasei Subsp. Tolerance and Lactobacillus Delbrueckii Subsp. Bulgaricus in Oral Capsules, Research in Pharmaceutical Sciences (2012)
14. The Anti-Biofouling Effect of Lactobacillus Fermentum-Derived Biosurfactant Against Streptococcus Mutans, Biofouling (2011)
15. Risk Assessment of the Growth Of Clostridium Botulinum And Spores Germination Induced by High Hydrostatic Pressure in Seafood, International Journal of Environmental Health Engineering (2016)
16. In Vitro Anti-Bacterial and Anti-Adherence Effects of Lactobacillus Delbrueckii Subsp Bulgaricus on Escherichia Coli, Research in Pharmaceutical Sciences (2013)
17. The Antibacterial Effects of Coffee Extract, Chlorhexidine, and Fluoride Against Streptococcus Mutans and Lactobacillus Plantarum: An in Vitro Study, Dental Research Journal (2019)
18. Evaluation of Response Surface Methodology to Predict Optimum Growth Conditions for Lactobacillus Plantarum A7 (Kc 355240) in Probiotic Soy Milk Containing Essential Oil of Cuminum Cyminum, Recent Patents on Food, Nutrition and Agriculture (2016)
19. Characterization and in Vitro Probiotic Assessment of Potential Indigenous Bacillus Strains Isolated From Soil Rhizosphere, Minerva Biotecnologica (2016)
20. Expression of Biologically Active Murine Interleukin-18 in Lactococcus Lactis, FEMS Microbiology Letters (2016)
21. The Antimicrobial Effects of Lactobacillus Plantarum and Lactobacillus Rhamnosus Isolated From Local Kefir Buttermilk on Oral Pathogens; [ثر ضد میکروبی الکتوباسیلوس پالنتاروم و الکتوباسیلوس رامنوزوس جداسازی شده از دوغ کفیر بومی اصفهانبر روی پاتوژنهای دهانی], Journal of Isfahan Medical School (2023)
22. Comparison of Antimicrobial and Antioxidant Effects of Sesame Oil Produced by Traditional, Industrial, and Cold Press Methods, Journal of Isfahan Medical School (2020)
23. Survey of Listeria Monocytogenes Contamination of Various Environmental Samples and Associated Health Risks, Food Control (2020)
24. Prophylactic Effects of Secretion Metabolites of Dairy Lactobacilli Through Downregulation of Erbb-2 and Erbb-3 Genes on Colon Cancer Cells, European Journal of Cancer Prevention (2020)
25. Performance of the Municipal Wastewater Treatment Plant for Removal of Listeria Monocytogenes, International Journal of Environmental Health Engineering (2013)
26. Antibacterial Effects of Hydrogen Peroxide and Silver Composition on Selected Pathogenic Enterobacteriaceae, International Journal of Environmental Health Engineering (2012)
27. The Assessment of Biofilm Formation in Iranian Meat Processing Environments, Research Journal of Microbiology (2008)
28. Protection of Lactoperoxidase Activity With Sugars During Lyophilization and Evaluation of Its Antibacterial Properties, Research in Pharmaceutical Sciences (2015)
29. Production and Evaluation of a Probiotic Yogurt Using Lactobacillus Casei Ssp. Casei, International Journal of Dairy Technology (2009)
30. Lactobacillus Plantarum A7, a Potential Probiotic Strain From Infant Fecal Flora, Journal of Biology and Today's World (2017)
31. Antimicrobial Potential of Probiotic Strains From Bulgarian Cheese and Shallot Yogurt Against Staphylococcus Saprophyticus, BioMed research international (2024)
32. Probiotic Lactobacillus and the Potential Risk of Spreading Antibiotic Resistance: A Systematic Review, Research in Pharmaceutical Sciences (2023)
33. Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia Coli Isolated From Milk and Dairy Products in Isfahan, Iran, Foods (2022)
34. Effects of Biosurfactants Derived From Lactobacillus Casei and Lactobacillus Reuteri on Gene Expression Profile of Gtfb/C and Ftf Genes in Lactobacillus Mutans, Journal of Isfahan Medical School (2014)
35. Lactobacillus Rhamnosus Biosurfactant Inhibits Biofilm Formation and Gene Expression of Caries-Inducing Streptococcus Mutans, Dental Research Journal (2019)
36. Prevalence of Listeria Species in Food Products in Isfahan, Iran, International Journal of Food Microbiology (2008)
37. Antimicrobial and Antibiofilm Effects of Cyclic Dipeptide-Rich Fraction From Lactobacillus Plantarum Loaded on Graphene Oxide Nanosheets, Frontiers in Microbiology (2024)
38. Microbial and Physiochemical Changes in Yoghurts Containing Different Lactobacillus Delbrueckii Subsp. Bulgaricus Strains in Association With Lactobacillus Plantarum As an Adjunct Culture, International Journal of Dairy Technology (2014)
39. Salivary Streptococcus Mutans and Lactobacilli Levels Following Probiotic Cheese Consumption in Adults: A Double Blind Randomized Clinical Trial, Journal of Research in Medical Sciences (2012)
40. Evaluation of the Simultaneous Effects of Lactobacillus Delbrueckii and Lactobacillus Lactis on Biofilms of Isolates From Chronic Ulcer Infections With Multiple-Drug Resistance, Jundishapur Journal of Microbiology (2022)
41. The Prevalence of Bacterial Contamination of Table Eggs From Retails Markets by Salmonella Spp., Listeria Monocytogenes, Campylobacter Jejuni and Escherichia Coli in Shahrekord, Iran, Jundishapur Journal of Microbiology (2011)
42. Prevalence Of Listeria Monocytogenes In the River Receiving the Effluent of Municipal Wastewater Treatment Plant, International Journal of Environmental Health Engineering (2013)
43. Epidemiology of Listeria Monocytogenes Prevalence in Foods, Animals and Human Origin From Iran: A Systematic Review and Meta-Analysis, BMC Public Health (2018)
44. Isolation and Purification of an Ultraviolet-Stable Bacteriocin Produced by Enterococcus Faecium Strain Dsh20 Against Listeria Monocytogenes, Journal of Isfahan Medical School (2013)
45. Enumeration of Lactobacilli in the Fecal Flora of Infant Using Two Different Modified De-Man Rogosa Sharpe Media Under Aerobic and Anaerobic Incubation, Pakistan Journal of Biological Sciences (2008)
46. Virulence Factors, Biofilm Formation and Antibiotic Resistance Pattern in Enterococcus Faecalis and Enterococcus Faecium Isolated From Clinical and Commensal Human Samples in Isfahan, Iran, Annali di Igiene Medicina Preventiva e di Comunita (2019)
47. Effects of Lactobacillus Plantarum A7 With Probiotic Potential on Colon Cancer and Normal Cells Proliferation in Comparison With a Commercial Strain, Iranian Journal of Basic Medical Sciences (2014)