Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium

Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium Publisher

Zolfaghar M^{1, 2} ; Mirzaeian L¹ ; Beiki B³ ; Naji T² ; Moini A⁴ ; Eftekhariyazdi P¹ ; Akbarinejad V⁵ ; Vernengo AJ⁶ ; Fathi R¹

Show Affiliations

1. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
2. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, IAUPS, Tehran, Iran
3. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
5. Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
6. Rowan University, Department of Biomedical Engineering, 201 Mullica Hill Rd, Glassboro, 08028, NJ, United States

Source: Heliyon Published:2020

Abstract

Wharton's jelly derived-mesenchymal stem cells (WJ-MSCs) have a same developmental origin with primordial germ cells. WJ-MSCs perhaps differentiate into oocyte and germ like-cells (OLCs/GLCs) in the presence of appropriate inducers. Human follicular fluid (FF) and cumulus cells conditioned medium (CCM) are naturally rich sources for oocyte development. The aim of this study was to evaluate WJ-MSCs potential for differentiating into OLCs and GLCs exposed to FF and CCM. WJ-MSCs were cultured in two different induction media (10% FF, 10% CCM) for 21 days. Morphological changes and expression of developmental genes were evaluated on days 0, 7, 14 and 21 of culture. Also, on 21st day of culture, the expression of oocyte and germ cell proteins investigated using immunofluorescence staining. Appearance of round shaped cells from 7th day onwards indicated that WJ-MSCs can differentiate into OLCs when exposed to FF and CCM. The size of produced OLCs and expression of oocyte specific genes and proteins were increased more positively in FF group rather than CCM group. Although, WJ-MSCs could differentiate into OLCs by FF and CCM, however, the induction potential of FF for producing OLCs was better than CCM. © 2020; Cell biology; Cell differentiation; Stem cells research; Embryology; Regenerative medicine; Wharton's jelly mesenchymal stem cells, Follicular fluid, Cumulus cells conditioned medium, Oocyte and germ like-cells © 2020

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Mehdi Abbasi (3)

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Style	Citing Format
MLA	Zolfaghar M, et al.. "Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium." Heliyon, vol. 6, no. 10, 2020, pp. -.
APA	Zolfaghar M, Mirzaeian L, Beiki B, Naji T, Moini A, Eftekhariyazdi P, Akbarinejad V, Vernengo AJ, Fathi R (2020). Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium. Heliyon, 6(10), -.
Chicago	Zolfaghar M, Mirzaeian L, Beiki B, Naji T, Moini A, Eftekhariyazdi P, Akbarinejad V, Vernengo AJ, Fathi R. "Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium." Heliyon 6, no. 10 (2020): -.
Harvard	Zolfaghar M et al. (2020) 'Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium', Heliyon, 6(10), pp. -.
Vancouver	Zolfaghar M, Mirzaeian L, Beiki B, Naji T, Moini A, Eftekhariyazdi P, et al.. Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium. Heliyon. 2020;6(10):-.
BibTex	@article{ author = {Zolfaghar M and Mirzaeian L and Beiki B and Naji T and Moini A and Eftekhariyazdi P and Akbarinejad V and Vernengo AJ and Fathi R}, title = {Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium}, journal = {Heliyon}, volume = {6}, number = {10}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Zolfaghar M AU - Mirzaeian L AU - Beiki B AU - Naji T AU - Moini A AU - Eftekhariyazdi P AU - Akbarinejad V AU - Vernengo AJ AU - Fathi R TI - Wharton's Jelly Derived Mesenchymal Stem Cells Differentiate Into Oocyte Like Cells in Vitro by Follicular Fluid and Cumulus Cells Conditioned Medium JO - Heliyon VL - 6 IS - 10 SP - EP - PY - 2020 ER -

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