Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells

Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells Pubmed

Ghasemi Hamidabadi H¹ ; Sobhani A² ; Nazm Bojnordi M¹

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1. Cellular and Molecular Research Center, Department of Anatomy and Cell Biology, Mazandaran University of Medical Sciences, Sari, Iran
2. Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran

Source: Archives of Iranian Medicine Published:2015

Abstract

Background: Stage-specific embryonic antigen- 1 (SSEA1) is a cell surface carbohydrate that its pattern expression is changed during induction of mouse embryonic stem cell differentiation. In this study, the spatial distribution of SSEA1 on primordial germ cells differentiation and subsequent progression into oocyte-like cells from mouse embryonic stem cells in vitro was evaluated. Methods: Embryoid bodies from mouse embryonic stem cells were cultured for two days with 5 ng/mL BMP4. SSEA-1 positive and negative cells were separated using the MACS system and cultured separately in a conditioned medium consist of in vitro maturation medium diluted in DMEM [1:1] for 10 days. We assayed viability, colony formation and alkaline phosphatase activity (ALP) of sorted cells. Also, germ cell markers were analyzed by flow cytometry, Immunocytochemistry and RT-PCR. Results: Viability percent SSEA-1 positive cells were more than SSEA-1 negative cells. SSEA-1 positive cells and SSEA-1 negative cells formed compact and flat colonies respectively. Unlike the SSEA-1 positive population, the SSEA-1 negative colonies showed a weakALP activity. SSEA-1 positive cells expressed Oct4, Stella, Mvh, c-kit, Scp3, Desmin, GFAP and Albumin. Interestingly, SSEA-1 negative cells expressed Desmin and GFAP. The population of Mvh-positive cells in SSEA-1 positive was 17.74%. All specific oocyte mentioned genes were detected in the SSEA-1 positive. Also, oocyte specific proteins GDF9 and ZP3 were detected using Immunocytochemistry. Conclusion: Our results suggest that conditioned medium provides a suitable niche to differentiation and progression putative primordial germ cells derived from the SSEA-1positive toward oocyte-like cells. © 2015, Academy of Medical Sciences of I.R. Iran. All rights reserved.

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Tayebeh Rastegar (3)

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Style	Citing Format
MLA	Ghasemi Hamidabadi H, Sobhani A, Nazm Bojnordi M. "Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells." Archives of Iranian Medicine, vol. 18, no. 7, 2015, pp. 404-410.
APA	Ghasemi Hamidabadi H, Sobhani A, Nazm Bojnordi M (2015). Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells. Archives of Iranian Medicine, 18(7), 404-410.
Chicago	Ghasemi Hamidabadi H, Sobhani A, Nazm Bojnordi M. "Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells." Archives of Iranian Medicine 18, no. 7 (2015): 404-410.
Harvard	Ghasemi Hamidabadi H, Sobhani A, Nazm Bojnordi M (2015) 'Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells', Archives of Iranian Medicine, 18(7), pp. 404-410.
Vancouver	Ghasemi Hamidabadi H, Sobhani A, Nazm Bojnordi M. Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells. Archives of Iranian Medicine. 2015;18(7):404-410.
BibTex	@article{ author = {Ghasemi Hamidabadi H and Sobhani A and Nazm Bojnordi M}, title = {Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells}, journal = {Archives of Iranian Medicine}, volume = {18}, number = {7}, pages = {404-410}, year = {2015} }
RIS	TY - JOUR AU - Ghasemi Hamidabadi H AU - Sobhani A AU - Nazm Bojnordi M TI - Multipotent Ssea-1 Positive Cells Population Differentiation Into Primordial Germ Cells and Subsequently Progress Into Oocyte-Like Cells JO - Archives of Iranian Medicine VL - 18 IS - 7 SP - 404 EP - 410 PY - 2015 ER -

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