Association of Tumour Necrosis Factor-Alpha G/A -238 and G/A -308 Single Nucleotide Polymorphisms With Juvenile Idiopathic Arthritis Publisher Pubmed



Maddah M¹ ; Harsini S^{2, 3} ; Ziaee V^{1, 4} ; Moradinejad MH¹ ; Rezaei A² ; Zoghi S^{3, 5} ; Sadr M⁶ ; Aghighi Y⁷ ; Rezaei N^{2, 3, 5, 6} Show Affiliations
Source: International Journal of Immunogenetics Published:2016

Abstract

Juvenile idiopathic arthritis (JIA) is a heterogeneous autoimmune disorder of unknown origin. As proinflammatory cytokines are known to contribute towards the pathogenesis of JIA, this case–control study was performed to examine the associations of certain single nucleotide polymorphisms (SNPs) of tumour necrosis factor-α (TNF-α) gene. Fifty-three patients with JIA participated in this study as patients group and compared with 137 healthy unrelated controls. Genotyping was performed for TNF-α gene at positions -308 and -238, using polymerase chain reaction with sequence-specific primers method. Results of the analysed data revealed a significant positive association for TNF-α gene at positions -308 and -238 for A allele in patients group compared with controls (P < 0.01). At the genotypic level, the frequency of TNF-α gene at positions -308 and -238 for GG genotype was discovered to be higher in the patients with JIA compared to the healthy controls (P < 0.01), while GA genotype at the same positions was observed to be less frequent in the case group than the controls (P < 0.01). At the haplotypic level, a significant positive association for TNF-α GG haplotype (positions -308, -238) together with a notable negative association for TNF-α AG and GA haplotypes at the same positions were detected in the patients group in comparison with the healthy individuals (P < 0.01). Cytokine gene polymorphisms might affect the development of JIA. Particular TNF-α gene variants could render individuals more susceptible to JIA. © 2016 John Wiley & Sons Ltd