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Construction of Metronidazole Capped in Gold Nanoparticles Against Helicobacter Pylori: Antimicrobial Activity Improvement; [Разработка Метронидазола, Покрытого Золотыми Наночастицами, Против Helicobacter Pylori: Улучшение Антимикробной Активности] Publisher Pubmed



Fateh R1 ; Javadi A2 ; Kardanyamch J2 ; Rahdar HA3 ; Amini M2 ; Ghasemi F2 ; Azimi A2 ; Davarpanah M2 ; Mohammadzadeh R2
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Qom University of Medical Sciences, Qom, Iran
  2. 2. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran

Source: Folia Medica Published:2021


Abstract

Introduction: Helicobacter pylori is considered a major agent causing gastritis and peptic ulcer disease. Unfortunately, the occurrence of increasing drug resistance to this bacterium would result in some difficulties in its treatment. Therefore, the application of nanotechnology has been suggested to resolve such problems. Nanoparticles usage in medical research has been expanded in recent years. Among nanometals, gold nanoparticles have exclusive features that can be used in such applications. Using nanotechnology in medical science could help mankind to solve this problem in the future. Aim: Our aim in this research was to investigate the antimicrobial effect of gold nanoparticles on H. pylori strains. Materials and methods: Gold nanoparticles were synthesized by the Turkevich method. Then, their size and dispersion were investigated using spectrophotometry, DLS, and TEM microscopy. Subsequently, the combination of metronidazole and gold nanoparticles was obtained by mixing method, and then the anti-helicobacter effects of the two were evaluated according to CLSI. Results: The highest size of gold nanoparticles was between 12 and 9 nm, and the maximum absorbance was 522 nm; however, in conjugated state, the maximum absorbance was 540 nm, which indicated the accumulation of drug-conjugated nanoparticles in the conjugate state. Some changes indicated the binding of metronidazole to gold nanoparticles. Antimicrobial testing of gold nanoparticles and metronidazole did not affect the Helicobacter pylori. Therefore, the combination of gold nanoparticles and metronidazole had a 17-mm growth inhibition zone. Conclusions: The anti-helicobacter effects of metronidazole significantly increased in conjugation with gold nanoparticles. © Folia Medica.