Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Diagnostic Value of Plasma Long Non-Coding Rna Hottip As a Non-Invasive Biomarker for Colorectal Cancer (A Case-Control Study) Publisher



Akbaresfahani SA1 ; Karimipoor M2 ; Bahreini F1 ; Soltanian AR3 ; Aletaha N4 ; Mahdavinezhad A5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Modelling of Non communicable Diseases Research Center, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
  4. 4. Department of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Science, Tehran, Iran
  5. 5. Research center for Molecular Medicine, Department of Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran

Source: International Journal of Molecular and Cellular Medicine Published:2019


Abstract

Long non-coding RNAs (lncRNAs), associated with various cancers including colorectal cancer (CRC), could be collected from body fluids easily. Our aims were to determine the expression level of HOTTIP lncRNA in plasma samples of healthy individuals and CRC patients as well as their relationship with clinico-pathological characteristics of patients. First, total RNA was extracted from the plasma samples of 100 subjects including 50 patients and 50 age and sex matched healthy persons. Then, gene expression was measured using real-time PCR technique. The sensitivity and specificity of HOTTIP dysregulation in CRC and healthy individual's plasma was measured by receiver operating characteristic (ROC) analysis. As compared with healthy controls, HOTTIP lncRNA was over expressed in a statistically significant manner in plasma samples of patients (P=0.001). Significant relationship between HOTTIP expression and positive family history of CRC was observed, too (P=0.04). The ROC curve analysis showed an AUC value of 0.775, a specificity of 82%, a sensitivity of 76%, with a cut off value equal to 2.40 (P =0.001). HOTTIP transcript can be proposed as a new biomarker for early diagnosis due to the increased expression in plasma samples of patients with CRC and the relatively high sensitivity and specificity. © 2019 Babol University of Medical Sciences.
Related Docs
1. Autophagy and Gastrointestinal Cancers: The Behind the Scenes Role of Long Non-Coding Rnas in Initiation, Progression, and Treatment Resistance, Cancer Gene Therapy (2021)
2. Association of Anril Expression With Coronary Artery Disease in Type 2 Diabetic Patients, Cell Journal (2018)
3. Clinicopathological and Prognostic Value of Lncrna Pandar Expression in Solid Tumors: Evidence From a Systematic Review and Meta-Analysis, Journal of Cellular Physiology (2019)
4. Overexpression of Purpl and Downregulation of Nonhsat062994 As Potential Biomarkers in Gastric Cancer, Life Sciences (2019)
5. New Insights Into the Long Non-Coding Rnas Dependent Modulation of Heart Failure and Cardiac Hypertrophy: From Molecular Function to Diagnosis and Treatment, Current Medicinal Chemistry (2024)
6. Long Non-Coding Rnas As Epigenetic Regulators in Cancer, Current Pharmaceutical Design (2019)
Experts (# of related papers)
Hamid Reza Mirzaei (2)
Mohammad Ali Boroumand (1)