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Aerobic Endurance Training Improves Nonalcoholic Fatty Liver Disease (Nafld) Features Via Mir-33 Dependent Autophagy Induction in High Fat Diet Fed Mice Publisher Pubmed



Ghareghani P1, 2 ; Shanaki M2 ; Ahmadi S3 ; Khoshdel AR4 ; Rezvan N5 ; Meshkani R3 ; Delfan M6 ; Gorganifiruzjaee S3, 7
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, Faculty of Biology, Islamic Azad University, Branch of Fars, Shiraz, Iran
  2. 2. Department of Medical Laboratory Sciences, School of Allied Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Epidemiology, School Medicine, AJA University of Medical sciences, Tehran, Iran
  5. 5. Department of Clinical Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, International Campus, Tehran, Iran
  6. 6. Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahar University, Tehran, Iran
  7. 7. Department of Medical Laboratory Sciences, School of Allied Health Medicine, AJA University of Medical sciences, Tehran, Iran

Source: Obesity Research and Clinical Practice Published:2018


Abstract

Due to changes in life style, obesity and obesity related complication such as insulin resistance, type 2 diabetes and non-alcoholic fatty liver disease caused worldwide health problems. Regular exercise has been frequently prescribed to combat metabolic complication of obesity but its molecular mechanism has not been fully illustrated. We investigated molecular mechanism of lipid lowering effect of exercise training in high fat diet fed mice by focusing on miR-33 expression and autophagy pathway. 24 mice were assigned to normal chow (NC) (n = 8), high-fat diet (HFD) (n = 16) group and subjected to NC and HFD for 13-weeks. HFD groups were divided to sedentary (HFD n = 8) or continuous endurance training (HFD + CET, n = 8) subgroups. The HFD + CET mice were subjected to treadmill running for 10-weeks in 23-week HFD course. HFD increased body weight, fasting blood sugar, triglyceride, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), liver lipogenic genes expression and reduced miR-33 mRNA expression and autopahgy pathway while training program reversed them. Exogenous miR-33 mimic sequence induced autophagy and reduced lipogenesis in HepG2 cells. Autophagy induction by rapamycin reduced lipogenesis and autophagy inhibition by chloroquine, enhanced lipogenesis in HepG2 cells. These findings suggest that aerobic exercise training as a non-pharmacological therapy exerts its lipid lowering effects by miR-33 dependent autophagy induction. © 2017 Asia Oceania Association for the Study of Obesity