Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B

Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B Publisher Pubmed

Zare M¹ ; Panahi G² ; Koushki M² ; Mostafavipour Z^{1, 3} ; Meshkani R²

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1. Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Archives of Physiology and Biochemistry Published:2022

Abstract

Introduction: Here, we aimed to investigate whether the beneficial effects of metformin on lipid accumulation is mediated through regulation of miR-33b. Methods: The expression of the genes and miRNAs and protein levels were evaluated using real-time PCR and western blot, respectively. To investigate the potential role of miR-33b in lipid accumulation, the mimic of the miR-33b was transfected into HepG2 cells. Results: We found that metformin reduces high glucose-induced lipid accumulation in HepG2 cells through inhibiting of SREBP1c and FAS and increasing the expression of CPT1 and CROT. Overexpression of miR-33b significantly prevented the decreasing effect of metformin on lipid content and intra and extra triglyceride levels. Importantly, miR-33b mimic inhibited the increasing effects of metformin on the expression of CPT1 and CROT. Conclusion: These findings suggest that metformin attenuates high glucose-induced lipid accumulation in HepG2 cell by downregulating the expression of miR-33b. © 2019 Informa UK Limited, trading as Taylor & Francis Group.

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Reza Meshkani (3)

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Style	Citing Format
MLA	Zare M, et al.. "Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B." Archives of Physiology and Biochemistry, vol. 128, no. 2, 2022, pp. 333-340.
APA	Zare M, Panahi G, Koushki M, Mostafavipour Z, Meshkani R (2022). Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B. Archives of Physiology and Biochemistry, 128(2), 333-340.
Chicago	Zare M, Panahi G, Koushki M, Mostafavipour Z, Meshkani R. "Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B." Archives of Physiology and Biochemistry 128, no. 2 (2022): 333-340.
Harvard	Zare M et al. (2022) 'Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B', Archives of Physiology and Biochemistry, 128(2), pp. 333-340.
Vancouver	Zare M, Panahi G, Koushki M, Mostafavipour Z, Meshkani R. Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B. Archives of Physiology and Biochemistry. 2022;128(2):333-340.
BibTex	@article{ author = {Zare M and Panahi G and Koushki M and Mostafavipour Z and Meshkani R}, title = {Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B}, journal = {Archives of Physiology and Biochemistry}, volume = {128}, number = {2}, pages = {333-340}, year = {2022} }
RIS	TY - JOUR AU - Zare M AU - Panahi G AU - Koushki M AU - Mostafavipour Z AU - Meshkani R TI - Metformin Reduces Lipid Accumulation in Hepg2 Cells Via Downregulation of Mir-33B JO - Archives of Physiology and Biochemistry VL - 128 IS - 2 SP - 333 EP - 340 PY - 2022 ER -

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